Brilliant Violet 421™ anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A2F10_BV421_CD135_Antibody_FC_1_072313.jpg
C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11b APC and CD135 (clone A2F10) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A2F10_BV421_CD135_Antibody_FC_1_072313.jpg
    C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11b APC and CD135 (clone A2F10) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A2F10_BV421_CD135_Antibody_FC_2_072313.jpg
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
135313 125 µl 132€
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135315 50 µg 184€
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135314 500 µl 292€
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This product is offered in multiple sizing options (µg and µL/test sizes). To compare these sizing options and learn which is the best choice for you, please contact tech support.
Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow and on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
µg size: 0.2 mg/ml
test sizes: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of the this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Esplin BL, et al. 2011. J. Immunol. 186:5367. PubMed
Product Citations
  1. Bellelli R 2018. Molecular cell. 70:707. PubMed
  2. Radulović V, et al. 2019. Cell Rep. 27:2826. PubMed
Publication Library
RRID
AB_2562338 (BioLegend Cat. No. 135313)
AB_2571919 (BioLegend Cat. No. 135315)
AB_2562339 (BioLegend Cat. No. 135314)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution
Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.
Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al. Nat. Immunol. 9:676.
2. Veiby OP, et al. 1996. Blood 88:1256.
3. Veiby OP, et al. 1996. J. Immunol. 157:2953.
4. Mattews W, et al. 1991. Cell. 65:1143.
5. Hannum C, et al. 1994. Nature 368:643.
6. Ogawa M, et al. 1998. Exp Hematol. 26:478.

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 02-25-2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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