APC anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
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105811 25 µg 56,00€
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105812 100 µg 140,00€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of acetone fixed frozen sections2. The 2B8 antibody does not block c-Kit activity.

Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
Product Citations
  1. N. H, et al. 2017. Journal of Vascular Research. 10.1159/000464287. PubMed
  2. Guo B, et al. 2018. Nat Med. 24:360. PubMed
  3. Gao B, et al. 2018. Aging Cell. 3. PubMed
  4. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  5. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  6. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
  7. Sun Y et al. 2018. Cancer cell. 34(4):643-658 . PubMed
  8. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  9. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  10. Zhu H, et al. 2018. Elife. 7:. PubMed
  11. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  12. Engblom C, et al. 2017. Science. 358:6367. PubMed
  13. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  14. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  15. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  16. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  17. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  18. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  19. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  20. Plein A, et al. 2018. Nature. 562:223. PubMed
  21. Sibon D et al. 2019. Cell reports. 26(12):3246-3256 . PubMed
  22. Convente MR, et al. 2018. J Bone Miner Res. 33:269. PubMed
  23. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  24. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  25. Brian BF 4th et al. 2019. Elife. 8 pii: e46043. PubMed
  26. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  27. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  28. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  29. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  30. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  31. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  32. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  33. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  34. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  35. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  36. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  37. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  38. Liu J, et al. 2012. Proc Natl Acad Sci U S A. 109:10018. PubMed
  39. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  40. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  41. Artinger E, et al. 2013. Proc Natl Acad Sci U S A. 110:12000. PubMed
  42. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  43. Umemoto T, et al. 2008. Biochem Biophys Res Commun. 365:176. PubMed
  44. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  45. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  46. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  47. Gandhapudi S, et al. 2015. J Immunol. 194:3820. PubMed
  48. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  49. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  50. Zhu M, et al. 2015. J Immunol. 195: 4492 - 4502. PubMed
  51. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  52. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  53. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  54. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  55. Ritter M, et al. 2020. Ann Hematol. 99:2329. PubMed
  56. Wang X, et al. 2020. Cell Res. 30:1109. PubMed
  57. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  58. Chen CC, et al. 2020. Cancer Cell. 37:71. PubMed
  59. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  60. Suzuki S, et al. 2021. Cell Reports. 34(7):108752. PubMed
  61. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  62. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  63. Inverso D, et al. 2021. Developmental Cell. 56(11):1677-1693.e10. PubMed
  64. Barkaway A, et al. 2021. Immunity. . PubMed
  65. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
RRID
AB_313220 (BioLegend Cat. No. 105811)
AB_313221 (BioLegend Cat. No. 105812)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06-28-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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