PE anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_A2F10_PE_102309
Bone marrow cells from C57BL/6 mouse stained with A2F10 PE and CD45R/B220 (RA3-6B2) FITC
  • A_A2F10_PE_102309
    Bone marrow cells from C57BL/6 mouse stained with A2F10 PE and CD45R/B220 (RA3-6B2) FITC
  • B_A2F10_PE_102309
    Bone marrow cells from C57BL/6 mouse stained with rat IgG2a, k PE and CD45R/B220 (RA3-6B2) FITC
See PE spectral data
Cat # Size Price Quantity Avail. Save
135305 50 µg 76€
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135306 200 µg 220€
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  2. Giotopoulos G, et al. 2015. J Exp Med. 212: 1551-1569. PubMed
  3. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  4. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  5. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  6. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  7. Jackson JT, et al. 2018. Blood Adv. 2:347. PubMed
  8. Kim SK, et al. 2018. Genes Dev. 32:849. PubMed
  9. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  10. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  11. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  12. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  13. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  14. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  15. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  16. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  17. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  18. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  19. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  20. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  21. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  22. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  23. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  24. Di Genua C, et al. 2020. Cancer Cell. 37:690. PubMed
  25. Delbridge AR, et al. 2017. Cell Death Dis. 8:e2914. PubMed
RRID
AB_1877218 (BioLegend Cat. No. 135305)
AB_1877217 (BioLegend Cat. No. 135306)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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