APC/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
  • TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
    C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
116223 25 µg 68€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  2. McKenney AS, et al. 2018. J Clin Invest. 128:789. PubMed
  3. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  4. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  5. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  7. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  8. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  9. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  10. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  11. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  12. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  13. Xu R, et al. 2018. Nat Med. 24:823. PubMed
  14. Han Y, et al. 2019. J Clin Invest. 130:26. PubMed
  15. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  16. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  17. Kalamakis G, et al. 2019. Cell. 176:1407. PubMed
  18. Wang L, et al. 2020. Nat Commun. 0.654166667. PubMed
  19. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  20. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  21. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  24. Ji G, et al. 2019. Bone Joint J. 101-B:108. PubMed
  25. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  26. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  27. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  28. Xu X, et al. 2011. Am J Physiol Regul Integr Comp Physiol. 300:R1115. PubMed
  29. Castiglioni A, et al. 2015. PLoS One. 10:128094. PubMed
  30. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  31. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  32. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  33. Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed
  34. Venugopal J, et al. 2020. Sci Rep. 10:16514. PubMed
  35. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  36. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  37. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  38. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  39. Lu SX, et al. 2021. Cell. . PubMed
RRID
AB_2137788 (BioLegend Cat. No. 116223)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02-01-2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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