Purified anti-mouse TER-119/Erythroid Cells Maxpar Ready Antibody anti-TER-119

Pricing & Availability

Clone: TER-119 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-76
Isotype: Rat IgG2b, κ
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Product Citations: publications

C57BL/6 mouse splenocytes mixed with red blood cells and stained with 147Sm-anti-CD45 (30-F11) and 162Dy-anti-TER-119 (TER-119). Data provided by DVS Sciences.

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116241

100 µg

95€

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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
Oudelaar AM, et al. 2020. Nat Commun. 2.348611111. PubMed
Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed

RRID

AB_2563789 (BioLegend Cat. No. 116241)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All TER-119 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse TER-119/Erythroid Cells	TER-119	FC
Biotin anti-mouse TER-119/Erythroid Cells	TER-119	FC
FITC anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC, IHC-F, IHC-P, IP, WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)	TER-119	FC, CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC, IHC-F, IHC-P, IP, WB, CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody	TER-119	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07-21-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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