APC anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
FN50_APC_042706
PMA+ionomycin-stimulated (5hours) human peripheral blood lymphocytes stained with FN50 APC
  • FN50_APC_042706
    PMA+ionomycin-stimulated (5hours) human peripheral blood lymphocytes stained with FN50 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
310909 25 tests 52,00€
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310910 100 tests 112,00€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2 and immunofluorescence microscopy3.

Application References
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Jeng MY, et al. 2018. J Exp Med. 215:51. PubMed
  2. Pan Y, et al. 2018. Proc Natl Acad Sci U S A. 115:992. PubMed
  3. Warthan MD, et al. 2018. Biol Reprod. 98:309. PubMed
  4. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  5. Hill ZB, et al. 2018. Nat Chem Biol. 14:112. PubMed
  6. Tiwarekar V, et al. 2018. J Virol. 92:17. PubMed
  7. Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
  8. Lee YG, et al. 2019. Nat Commun. 10:2681. PubMed
  9. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  10. van Galen P, et al. 2019. Cell. 176:1265. PubMed
  11. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  12. Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed
  13. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  14. Paraiso HC, et al. 2018. J Neuroinflammation. 0.694444444. PubMed
  15. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  16. Seamon K, et al. 2020. Front Immunol. 11:396. PubMed
  17. Huang Z, et al. 2020. Sci Adv. 6:eaay9209. PubMed
  18. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  19. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  20. Li H, et al. 2020. Front Oncol. 1.477083333. PubMed
  21. Huang C, et al. 2020. Theranostics. 10:10498. PubMed
  22. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  23. Fiorillo E, et al. 2010. J Biol Chem. 285:26506. PubMed
  24. Søgaard O, et al. 2015. PLoS One. 11: 1005142. PubMed
  25. Urbanska K, et al. 2015. Cancer Immunol Res. 3: 1130 - 1137. PubMed
  26. Couturier J, et al. 2016. Retroviology. 13:30. PubMed
  27. Neidleman J, et al. 2020. Elife. 9:00. PubMed
  28. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  29. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  30. Cai J, et al. 2021. eLife. 10:00. PubMed
  31. Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
  32. Luengo A, et al. 2020. Molecular Cell. 81(4):691-707.e6. PubMed
  33. Dijkman K, et al. 2021. Cell Reports Medicine. 2(1):100187. PubMed
  34. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  35. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  36. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
RRID
AB_314844 (BioLegend Cat. No. 310909)
AB_314845 (BioLegend Cat. No. 310910)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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