Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody

Pricing & Availability
Clone
MAb414 (See other available formats)
Regulatory Status
RUO
Other Names
107 kD nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kD, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98
Isotype
Mouse IgG1
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Product Citations
publications
Mab414_A647_Nuclear_Pore_Complex_Antibody_012618
HeLa cells were fixed with methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ Isotype control antibody (Negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 Nuclear Pore Complex (Clone Mab414) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The images were captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations of primary antibodies for (A-B) are 2 µg/ml (1:1000 dilution), (C) is 1 µg/ml (1:500 dilution) and (D) is 0.5 µg/ml (1:250 dilution).
  • Mab414_A647_Nuclear_Pore_Complex_Antibody_012618
    HeLa cells were fixed with methanol for 10 minutes and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ Isotype control antibody (Negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 Nuclear Pore Complex (Clone Mab414) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The images were captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations of primary antibodies for (A-B) are 2 µg/ml (1:1000 dilution), (C) is 1 µg/ml (1:500 dilution) and (D) is 0.5 µg/ml (1:250 dilution).
  • Mab414_A647_Nuclear_Pore_Complex_Antibody_112618
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ Isotype control antibody (Negative, Cat. No. 400136) or (B-D) Alexa Fluor® 647 Nuclear Pore Complex (Clone Mab414) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations of primary antibodies for (A-B) are 5 µg/ml, (C) is 2 µg/ml and (D) is 1 µg/ml.
  • Mab414_A647_Nuclear_Pore_Complex_Antibody_2_012618
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 647 Mouse IgG1, κ Isotype control antibody (Negative, Cat. No. 400136) (B) Alexa Fluor® 647 Nuclear Pore Complex (Clone Mab414) or (C) Purified Nuclear Pore Complex (Clone Mab414) and Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-C) is 1/5. Concentration of primary antibodies for (A-C) is 2 µg/ml. Concentrations of secondary antibody for (C) is 2 µg/ml.
  • Mab414_A647_Nuclear_Pore_Complex_Antibody_3_081219
    Jurkat cells were fixed, permeabilized and intracellularly stained with anti-Nuclear Pore Complex Proteins (clone MAb414) Alexa Fluor® 647 (closed histogram), or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
See Alexa Fluor® 647 spectral data
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682203 25 µg 92,00€
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682204 100 µg 260,00€
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Description

Nuclear pores are large protein complexes that cross the nuclear envelope. The proteins that make up the nuclear pore complex are known as nucleoporins. About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains. Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins). The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e. they are highly flexible proteins that lack ordered secondary structure. These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats.

Nuclear pore complexes allow the transport of molecules across the nuclear envelope. This transport includes RNA and ribosomal proteins moving from nucleus to the cytoplasm and proteins (such as DNA polymerase and lamins), carbohydrates, signaling molecules and lipids moving into the nucleus. Although smaller molecules simply diffuse through the pores, larger molecules may be recognized by specific signal sequences and then be diffused with the help of nucleoporins into or out of the nucleus.. Each of the eight protein subunits surrounding the actual pore (the outer ring) projects a spoke-shaped protein over the pore channel.

Nucleoporin p62 (p62) protein remains associated with the nuclear pore complex-lamina fraction. p62 is synthesized as a soluble cytoplasmic precursor of 61 kDa followed by modification that involve addition of N-acetylglucosamine residues, followed by association with other complex proteins.The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

P62 is a serine/threonine rich protein of ~520 amino acids, with tetrapeptide repeats on the amino terminus and a series of alpha-helical regions with hydrophobic heptad repeats. P62 assembles into a complex containing 3 addition proteins, p60, p54 and p45 forming the p62 complex of ~235 kDa. Glycosylation appears to be involved in the assembly and disassembly of p62 into higher order complexes, and a serine/threonine rich linker region between Ser270 to Thr294 appear to be regulatory. The p62 complex is localized to both the nucleoplasmic and cytoplasmic sides of the pore complex and the relative diameter of p62 complex relative to the nuclear pore complex suggests it interacts in pore gating.

Antibodies to p62 complex are involved in 1 or more autoimmune diseases. P62 glycosylation is increased in diabetes. p62 is also more frequent in Stage IV primary biliary cirrhosis and is prognostic for severe disease. Reduced p62 production has been linked to Alzheimer's disease. It is thought oxidative damage of the p62 promoter is correlated with AD and other neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Vertebrate, Xenopus, Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The antibody was raised using a nuclear pore complex mixture.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
ICFC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250-1:500 dilution) is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.

*Predicted MW = 62 kD

MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. This antibody is believed to recognize the repeated FXFG repeat sequence in nuceloporins including p62, p152 and p90 and other proteins.

Application References
  1. Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
  2. Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
  3. Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
  4. Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
  5. Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
  6. Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
  7. Davis LI, Blobel G. 1986. Cell. 45:699. 
  8. Blobel G. 1985. PNAS USA 82:8527.
Product Citations
  1. Piracha ZZ, et al. 2020. J Virol. 94:00:00. PubMed
RRID
AB_2728507 (BioLegend Cat. No. 682203)
AB_2728508 (BioLegend Cat. No. 682204)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References
  1. Yoshimura S, et al. 2013. J. Cell Sci. 126:3141-50.
Gene ID
NA
UniProt
View information about Nuclear Pore Complex Proteins on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 08-12-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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