Purified anti-Nuclear Pore Complex Proteins Antibody (Previously Covance catalog# MMS-120P)

Pricing & Availability
Clone
MAb414 (See other available formats)
Regulatory Status
RUO
Other Names
107 kD nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kD, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98
Previously
Covance Catalog# MMS-120P
Isotype
Mouse IgG1
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Product Citations
publications
1_MAb414_Pure_NPCP_Antibody_111417
Immunofluorescence of HeLa with Nuclear Pore Complex Proteins Mouse primary antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). Exposure time is 80 ms. Concentration is 1 µg/ml.
  • 1_MAb414_Pure_NPCP_Antibody_111417
    Immunofluorescence of HeLa with Nuclear Pore Complex Proteins Mouse primary antibody. Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). Exposure time is 80 ms. Concentration is 1 µg/ml.
  • 2_MAb414_Pure_NPCP_Antibody_ICC_122117
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Nuclear Pore Complex (Clone Mab414) overnight at 4°C followed by Alexa Fluor® 647 (Red) Goat anti-Mouse IgG (Cat. No. 405322) incubation for one hour at room temperature. The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/5. Concentrations for (A-B) is 5 µg/ml, (C) is 2 µg/ml and (D) is 0.5 µg/ml.
  • 3_MAb414_Pure_NPCP_Antibody_2_111417
    Western blot analysis of cell lysates from HaCaT, Daudi (Human), Raw264.7 (Mouse) and UMR106 (Rat) using Nuclear Pore Complex Proteins Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306). GAPDH (Cat. No. 919501) was used as a loading control.
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902907 25 µL 60,00€
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902901 100 µL 172,00€
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902902 500 µL 332,00€
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Description

Nuclear pores are large protein complexes that cross the nuclear envelope. The proteins that make up the nuclear pore complex are known as nucleoporins. About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains. Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins). The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e. they are highly flexible proteins that lack ordered secondary structure. These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats.

Nuclear pore complexes allow the transport of molecules across the nuclear envelope. This transport includes RNA and ribosomal proteins moving from nucleus to the cytoplasm and proteins (such as DNA polymerase and lamins), carbohydrates, signaling molecules and lipids moving into the nucleus. Although smaller molecules simply diffuse through the pores, larger molecules may be recognized by specific signal sequences and then be diffused with the help of nucleoporins into or out of the nucleus.. Each of the eight protein subunits surrounding the actual pore (the outer ring) projects a spoke-shaped protein over the pore channel.

Nucleoporin p62 (p62) protein remains associated with the nuclear pore complex-lamina fraction. p62 is synthesized as a soluble cytoplasmic precursor of 61 kDa followed by modification that involve addition of N-acetylglucosamine residues, followed by association with other complex proteins.The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.

P62 is a serine/threonine rich protein of ~520 amino acids, with tetrapeptide repeats on the amino terminus and a series of alpha-helical regions with hydrophobic heptad repeats. P62 assembles into a complex containing 3 addition proteins, p60, p54 and p45 forming the p62 complex of ~235 kDa. Glycosylation appears to be involved in the assembly and disassembly of p62 into higher order complexes, and a serine/threonine rich linker region between Ser270 to Thr294 appear to be regulatory. The p62 complex is localized to both the nucleoplasmic and cytoplasmic sides of the pore complex and the relative diameter of p62 complex relative to the nuclear pore complex suggests it interacts in pore gating.

Antibodies to p62 complex are involved in 1 or more autoimmune diseases. P62 glycosylation is increased in diabetes. p62 is also more frequent in Stage IV primary biliary cirrhosis and is prognostic for severe disease. Reduced p62 production has been linked to Alzheimer's disease. It is thought oxidative damage of the p62 promoter is correlated with AD and other neurodegenerative disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Vertebrate, Xenopus, Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The antibody was raised using a nuclear pore complex mixture.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ICC - Quality tested
WB - Verified
IHC-F, IP, IEM - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1 μg/ml (1:1000 - 1:2000 dilution) is recommended. For Western blotting, the suggested use of this reagent is 0.2 - 0.5 µg per ml (1:2000 - 1:5000). For immunohistochemistry, a dilution of 1:500 is suggested. For immunoprecipitation and electron microscopy, the suggested dilution of this reagent is 1:5000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully with frozen sections.

*Predicted MW = 62 kD

MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. This antibody is believed to recognize the repeated FXFG repeat sequence in nuceloporins including p62, p152 and p90 and other proteins.

Application References
  1. Zheng X, et al. 2012. J. Biol. Chem. 287:38254. (IF) PubMed
  2. Kimura T, et al. 2003. Mol Cell Biol. 23:1304. (IHC) PubMed
  3. Lopez-Soler RI, et al. 2001. J Cell Biol. 154:61. (IF, WB) PubMed
  4. Aris JP, Blobel G. 1989. J Cell Biol. 108:2059. (WB, IP, IF, EM) PubMed
  5. Davis LI, Blobel G. 1987. PNAS USA. 84:7552. (IP, IF) PubMed
  6. Edens LJ, Levy DL. 2014. J. Cell. Biol. 206:473.
  7. Davis LI, Blobel G. 1986. Cell. 45:699.
  8. Blobel G. 1985. PNAS USA 82:8527.
Product Citations
  1. Urbanek M, Michalak M, Krzyzosiak W 2017. Methods. 10.1016/j.ymeth.2017.04.002. PubMed
  2. Fichtman B, et al. 2019. Nat Commun. 10:605. PubMed
  3. Dilsaver MR, et al. 2018. Mol Biol Cell. :mbcE18050277. PubMed
  4. Rosso G, et al. 2019. Adv Sci (Weinh). 6:1801638. PubMed
  5. Watters K, et al. 2017. J Virol. 91:. PubMed
  6. Patil H, et al. 2019. Small GTPases. 10:146. PubMed
  7. Sołtysik K, et al. 2019. Nat Commun. 10:473. PubMed
  8. Cianciolo Cosentino C, et al. 2019. Sci Rep. 9:4750. PubMed
  9. Ahn JH, et al. 2019. Exp Mol Med. 51:64. PubMed
  10. Wild P, et al. 2019. F1000Res. 8:198. PubMed
  11. Bonnin E, et al. 2018. PLoS Genet. 14:e1007845. PubMed
  12. Behrens RT, et al. 2017. J Virol. 91. PubMed
  13. Liu Z et al. 2019. Neuron. 102(3):587-601 . PubMed
  14. Martino L et al. 2017. Developmental cell. 43(2):157-171 . PubMed
  15. Magre I, et al. 2019. J Cell Sci. 132:jcs224501. PubMed
  16. Fichtman B, et al. 2019. Am J Hum Genet. 105:48:00. PubMed
  17. Kuhn TM, et al. 2019. J Cell Biol. 218:2945. PubMed
  18. Ben-Yishay R, et al. 2019. J Cell Biol. 218:2962. PubMed
  19. Chen P, et al. 2019. J Cell Biol. 218:4063. PubMed
  20. Ohkura M 2015. Genes Dev. 29: 1789-1794. PubMed
  21. Raghunayakula S, et al. 2015. PLoS One. 10: 0144508. PubMed
  22. Zheng X, et al. 2012. J Biol Chem. 287:38254-38264. PubMed
  23. Giacomini C, et al. 2016. Mol Biol Cell. 27: 35 - 47. PubMed
  24. Berneking L, et al. 2016. PLoS Pathog. 12: 1005660. PubMed
  25. Mimura Y, et al. 2016. J Cell Sci. 129: 4200 - 4212. PubMed
  26. Alves N, et al. 2017. Sci Rep. 7:41519. PubMed
  27. Heijo H, et al. 2020. Mol Biol Cell. 31:2703. PubMed
  28. He Y, et al. 2021. eLife. 10:00. PubMed
  29. Wigington CP, et al. 2020. Molecular Cell. 79(2):342-358.e12. PubMed
  30. Duan T, et al. 2021. Current Biology. 31(7):1450-1462.e3. PubMed
  31. Dewi FRP, et al. 2020. Autophagy. 1:. PubMed
  32. Mukherjee RN, et al. 2020. Dev Cell. 395:54. PubMed
  33. Ding B, et al. 2021. J Neurosci. 41:2024. PubMed
RRID
AB_2734672 (BioLegend Cat. No. 902907)
AB_2565026 (BioLegend Cat. No. 902901)
AB_2565027 (BioLegend Cat. No. 902902)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References

1. Yoshimura S, et al. 2013. J. Cell Sci. 126:3141-50.

Gene ID
NA
UniProt
View information about Nuclear Pore Complex Proteins on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11-14-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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