- Clone
- A21014A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ABL Proto-Oncogene 1 Nonreceptor tyrosine kinase; P150; C-ABL; Aberson Tyrosine-Protein kinase 1; ABL; CHDSKM
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a. A21014A_PURE_ABL1-Phospho_WB_100523 a. A21014A_PURE_ABL1-Phospho_WB_100523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A21014A_PURE_ABL1-Phospho_WB_100523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg total protein) from K562 cells untreated or treated with a Pan-Bcr-Abl inhibitor (AP 24534) (50 nM, 4 hours), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-ABL1 Phospho (Tyr245) (clone A21014A) (left), and anti-Pan ABL1 (right) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
K562 cells untreated (positive control, filled histogram), or treated with a Pan-BCR-ABL inhibitor (AP24534) (negative control, open histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with purified anti-ABL1 Phospho (Tyr245) (clone A21014A), or purified mouse IgG2b, κ isotype control (Cat. No. 400301) (open histogram, dashed line), followed by PE goat anti-mouse IgG (Cat. No. 405307).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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618651 | 25 µg | 141€ | ||||
618652 | 100 µg | 352€ |
ABL1 is a protooncogene that encodes a tyrosine kinase protein. ABL1 is involved in multiple cellular processes such as adhesion, cell division, autophagy, differentiation and response to stress. ABL1 kinase activity is negatively regulated by interaction with its SH3 domain, thus deletion of the SH3 domain results in an oncogene. Chromosomal translocation involving ABL1 has been found in various leukemias, where the most notable is the t (9;22) that results in a fusion with the breakpoint cluster region gene (BCR). This rearrangement is present in many cases of chronic myelogenous leukemia, and leads to an unregulated tyrosine kinase activity which induces uncontrolled cellular proliferation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human ABL1 phosphorylated at Tyr245
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For ICFC, cells were fixed and permeabilizes using Cyto-Fast™ Buffer system.
- RRID
-
AB_3068060 (BioLegend Cat. No. 618651)
AB_3068060 (BioLegend Cat. No. 618652)
Antigen Details
- Structure
- ABL1 is an approximately 125 kD protein. However, when it is fused with BCR, the molecular weight is around 210 kD.
- Distribution
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Mainly located in cytoplasm, but it can also be found in the nucleus, mitochondria and endoplasmic reticulum
- Function
- ABL1 is a protein kinase that is negatively regulated via interaction with its SH3 domain, and after deletion of the SH3 domain, it becomes an oncogene. It also plays a role in several cellular processes like: cell growth, cell motility, adhesion, autophagy, and apoptosis.
- Interaction
- Interacts with the network of fibers called the actin cytoskeleton
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology
- Molecular Family
- Phospho-Proteins
- Antigen References
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- Burslem GM, et al. 2019. Cancer Res. 79:4744-4753.
- Piedimonte M, et al. 2019. BMC Cancer. 19:50.
- Wylie AA, et al. 2017. Nature. 543:733-737.
- Gene ID
- 25 View all products for this Gene ID
- UniProt
- View information about ABL1 Phospho (Tyr245) on UniProt.org
Related FAQs
Other Formats
View All ABL1 Phospho (Tyr245) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-ABL1 Phospho (Tyr245) | A21014A | WB,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-ABL1 Phospho (Tyr245)
Whole cell extracts (15 µg total protein) from K562 cells un... K562 cells untreated (positive control, filled histogram), o...
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