- UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56;
- Other Names
- Lin 1
- Mouse IgG1, κ; Mouse IgG2b, κ
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- Product Citations
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This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.Product Details
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
View more applications data for this product in our Scientific Poster Library.
- Application References
- Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC)
- Doherty TA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:577. PubMed.
- Product Citations
AB_10612570 (BioLegend Cat. No. 348801)
T cells, B cells, NK cells, monocytes/macrophages, neutrophils
- Cell Type
- B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Gene ID
- 929 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 916 View all products for this Gene ID 2214 View all products for this Gene ID 4684 View all products for this Gene ID
- View information about Lineage on UniProt.org
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