- 93 (See other available formats)
- Other Names
- Fcγ R III/II, Ly-17
- Rat IgG2a, λ
- Ave. Rating
- 5 reviews
- Product Citations
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
- Recommended Usage
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.
(PubMed link indicates BioLegend citation)
- Oliver AM, et al. 1999. Hybridoma 18:113.
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
- Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
- Flores M, et al. 2008. FASEB J. 22:3661. PubMed
- Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
- Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
- Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
- Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
- Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
- Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
- Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
- Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
- Gillis TP, et al. 2014. Infect Immun. 82:3900. PubMed
- Minkah N, et al. 2015. J Virol. 89:3366. PubMed
- Product Citations
AB_1574973 (BioLegend Cat. No. 101319)
AB_1574975 (BioLegend Cat. No. 101320)
- Ig superfamily, 40-60 kD
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Low affinity receptors for IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- View information about CD16/32 on UniProt.org
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