FITC anti-mouse CD16/32 Antibody

Pricing & Availability
Clone
93 (See other available formats)
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
2 reviews
Product Citations
publications
93_FITC_051906
C57BL/6 mouse splenocytes stained with 93 FITC
  • 93_FITC_051906
    C57BL/6 mouse splenocytes stained with 93 FITC
See FITC spectral data
Cat # Size Price Save
101305 50 µg ¥14,000
101306 500 µg ¥48,000
Description

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sorted pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.

The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (≤1.0 µg per 106 cells in 100 µl volume) for 5-10 minutes on ice prior to immunostaining. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101310). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Personal communication (IP)
  2. Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
  3. Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
  4. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
  5. Clements JL, et al. 2006. J. Immunol. 177:905.
  6. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
  7. Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
  8. Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
  9. Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
  10. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  11. Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
  12. Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
  13. Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
  14. Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
  15. Dutow P, et al. 2014. J Infect Dis. PubMed
  16. Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
  17. Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
  18. Takei S, et al. 2014. Vaccine. 32:3066. PubMed
  19. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  20. Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
  21. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
  22. Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
  23. Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations
  1. Spillmann F, et al. 2007. J Immunol. 179:6395. PubMed
  2. Lebert-Ghali C, et al. 2010. Exp Hematol. 38:1074. PubMed
  3. Qiu J, et al. 2016. Sci Rep. 6:39407. PubMed
  4. Yamamoto R 2018. Cell stem cell. 22:600. PubMed
  5. Macdougall CE 2018. Cell metabolism. 27:588. PubMed
  6. Zhu YP 2018. Cell reports. 24:2329. PubMed
Publication Library
RRID
AB_312804 (BioLegend Cat. No. 101305)
AB_312805 (BioLegend Cat. No. 101306)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.

Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/23/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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