- 93 (See other available formats)
- Other Names
- Fcγ R III/II, Ly-17
- Rat IgG2a, λ
- Ave. Rating
- 1 reviews
- Product Citations
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.Product Details
- Antibody Type
- Host Species
- Sorted pre-B cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2.
The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (≤1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 μm filtered).
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Personal communication (IP)
- Oliver AM, et al. 1999. Hybridoma 18:113. (Block)
- Brummel R and Lenert P. 2005. J. Immunol. 174:2429.
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block)
- Clements JL, et al. 2006. J. Immunol. 177:905.
- Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed
- Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed
- Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed
- Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed
- Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
- Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed
- Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed
- Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed
- Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed
- Dutow P, et al. 2014. J Infect Dis. PubMed
- Fan Y, et al. 2014. J Exp Med. 211:313. PubMed
- Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed
- Takei S, et al. 2014. Vaccine. 32:3066. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed
- Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
- Everad A, et al. 2014. Nat Commun. 5:5648. PubMed
- Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
- Product Citations
AB_1877268 (BioLegend Cat. No. 101323)
AB_1877267 (BioLegend Cat. No. 101324)
- Ig superfamily, 40-60 kD
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Low affinity receptors for IgG
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Unkeless JC. 1989. J. Clin. Invest. 83:355.
3. Qiu WQ, et al. 1990. Science 248:732.
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- View information about CD16/32 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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