Purified anti-human IFN-γ Antibody

Pricing & Availability
MD-1 (See other available formats)
Regulatory Status
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Mouse IgG1, κ
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Product Citations
Cat # Size Price Save
507501 50 µg ¥23,320
507502 500 µg ¥62,040

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The MD-1 antibody reacts with human and monkey interferon-gamma (IFN-γ). The MD-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C.

ELISA Capture - Quality tested
ICFC, IHC-F, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 µg/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1,2,5 and ELISPOT3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).
Additional reported applications (for the relevant formats) include: Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Van der Meide P, et al. 1985. J. Immuno. Meth. 79:293.
  2. Yazdanbakhsh M, et al. 1993. Eur. J. Immuno. 23:3312.
  3. Besouw N, et al. 1994. J. Med. Primatol. 23:42.
  4. Van der Meide P, et al. 1995. J. Med. Primatol. 24:271.
  5. Hilkens C, et al. 1996. J. Immunol. 156:1722.
  6. Hamann D, et al. 1996. J. Immunol. 156:1387.
  7. Wassenaar A, et al. 1995. Infect. Immunity. 63:2147.
Product Citations
  1. Posseme C, et al. 2022. Cell Rep. 39:110989. PubMed
  2. Haug V, et al. 2020. Transplantation. e260:104. PubMed
  3. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  4. Huanosta-Murillo E, et al. 2021. Front Immunol. 12:668369. PubMed
AB_2122340 (BioLegend Cat. No. 507501)
AB_2122339 (BioLegend Cat. No. 507502)

Antigen Details

Cytokine; dimer; 20-25 kD (Mammalian)
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IFN-γ MD-1 ELISA Capture,ICFC,IHC-F,WB
Ultra-LEAF™ Purified anti-human IFN-γ MD-1 ELISA Capture,ICFC,IHC-F,WB
Go To Top Version: 3    Revision Date: 05/23/2013

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human IFN-γ

  • Ultra-LEAF™ Purified anti-human IFN-γ


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