PerCP Streptavidin

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Other Names
SAv-PerCP
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405213 100 µg ¥45,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP is useful for detecting biotinylated antibodies. The excitation of PerCP by 488 nm laser light induces a light emission maximum of 675 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-PerCP solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-PerCP is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PerCP is 120 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed
  2. Liao G, et al. 2012. Gastroenterology. 142:582. PubMed
  3. Smith KA, et al. 2012. Infect Immun. 80:3481. PubMed
  4. Li J, et al. 2013. Am J respir Cell Mol Biol. 48:314. PubMed
  5. Michael A, et al. 2013. J. Immunol. 190:5534. PubMed
  6. Tsiganov EN, et al. 2014. J Immunol. 192:4718. PubMed
  7. Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed
Product Citations
  1. Mara-Koosham G, et al. 2011. Infect Immun. 79:1770. PubMed
  2. Liao G, et al. 2012. Gastroenterology. 142:582. PubMed
  3. Smith K, et al. 2012. Infect Immun . 80:3481. PubMed
  4. Li J, et al. 2013. Am J Respir Cell Mol Biol. 48:314. PubMed
  5. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
  6. Tsiganov E, et al. 2014. J Immunol. 192:4718. PubMed
  7. Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed
  8. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  9. Jones M, et al. 2016. PLoS One. 11: 0157271. PubMed
  10. Klein H, et al. 2016. J Neuropathol Exp Neurol. 75: 1031 - 1047. PubMed
  11. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  12. González-Tajuelo R, et al. 2017. Sci Rep. 7:41841. PubMed
  13. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  14. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  15. Seong J, et al. 2018. Development. 145:14. PubMed
  16. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Go To Top Version: 4    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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