PE Streptavidin

Pricing & Availability
Other Names
Streptavidin-Phycoerythrin, SAv-PE
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Product Citations
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SAV_PE_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
  • SAV_PE_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
See PE spectral data
Cat # Size Price Save
405203 100 µg ¥9,000
405204 500 µg ¥32,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-PE solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 360 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
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Product Citations
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  33. Niki T, et al. 2009. J Biol Chem. 284:32344. PubMed
  34. Chappaz S, et al. 2010. J Immunol. 185:3514. PubMed
  35. Scatizzi J, et al. 2012. J Immunol. 188:3307. PubMed
  36. Yamakawa N, et al. 2012. Int Immunol. 25:45. PubMed
  37. Baccala R, et al. 2012. J Immunol. 189:5976. PubMed
  38. Grevers L, et al. 2013. Ann Rheum Dis. 72:278. PubMed
  39. Woo S, et al. 2013. J Leukoc Biol. 93:363. PubMed
  40. Ashbaugh J, et al. 2013. J Immunol. 190:4525. PubMed
  41. Reading J, et al. 2013. J Immunol. 190:4542. PubMed
  42. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
  43. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
  44. Tsukamoto H, et al. 2013. Biochem Biophys Res Commun. 440:31. PubMed
  45. Günaydın G, et al. 2014. Vaccine. 32:470. PubMed
  46. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  47. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  48. He X, et al. 2014. J Immunol. 193:1314. PubMed
  49. Wang W, et al. 2014. Proc Natl Acad Sci U S A. 111:14466. PubMed
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  52. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  53. Phiwpan K, et al. 2015. PLoS One. 10:128198. PubMed
  54. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
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Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 6    Revision Date: 06/17/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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