FITC Streptavidin

Pricing & Availability
Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
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Product Citations
publications
SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
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Cat # Size Price Save
405201 100 µg ¥7,000
405202 500 µg ¥20,000
Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Schmutz S, et al. 2009. J. Immunol. 183:2217. PubMed
  2. Cho HY, et al. 2009. Immunol Lett. 127:39. PubMed
  3. de Wispelaere D, et al. 2012. J. Virol. 86:7072. PubMed
  4. Fukuoka A, et al. 2013. Int Immunol. PubMed
  5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
  6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed
Product Citations
  1. Schmutz S, et al. 2009. J Immunol. 183:2217. PubMed
  2. Lin Y, et al. 2012. PLoS One. 7:e30507. PubMed
  3. Yang M 2012. J Virol. 86:7072. PubMed
  4. Fukuoka A, et al. 2013. Int Immunol. 25:373. PubMed
  5. Cho H, et al. 2009. Immunol Lett. 127:39. PubMed
  6. Giltiay N, et al. 2013. J Exp Med. 210:2773. PubMed
  7. Lee J, et al. 2014. J Rheumatol. 41:2425. PubMed
  8. Matt P, et al. 2015. PLoS One. 10: 0137474. PubMed
  9. Mchedlidze T, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.20. PubMed
  10. SL C, et al. 2016. JCI Insight. 1: 83116. PubMed
  11. Vigeland C, et al. 2016. PLoS One. 11: 0163288. PubMed
  12. Li S, et al. 2016. PLoS One. 11: 0163893. PubMed
  13. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  14. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  15. Sasaki N, Itakura Y, Toyoda M 2017. Stem Cell Res Ther. 10.1186/s13287-017-0534-1. PubMed
  16. Zhu Z, et al. 2017. Int J Biochem Cell Biol. 10.1016/j.biocel.2017.04.003. PubMed
  17. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  18. Hensel JA, et al. 2019. Mol Ther Oncolytics. 12:41. PubMed
  19. Mödinger Y, et al. 2018. J Cell Mol Med. 22:6002. PubMed
  20. Zhang Y, et al. 2019. JCI Insight. 4:2. PubMed
  21. Krishack PA, et al. 2019. JCI Insight. 4:6. PubMed
  22. Laurin M, et al. 2019. Elife. 8. PubMed
  23. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  24. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  25. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  26. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed

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Go To Top Version: 5    Revision Date: 06/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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