PE anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
4S.B3
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and 4S.B3 PE
  • 4S.B3
    PMA/Ionomycin-stimulated human PBMCs were stained with CD3 PE/Cy5 and 4S.B3 PE
Compare all formats See PE spectral data
Cat # Size Price Save
502508 25 tests ¥24,200
502510 50 µg ¥36,300
502509 100 tests ¥43,480
Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Ishii H, et al. 2016. Sci Rep. 6:30153. PubMed
  2. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  3. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  4. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  5. Hermans L, et al. 2021. Front Immunol. 634402:12. PubMed
  6. Chen L, et al. 2018. Immunology. 155:123. PubMed
  7. Tang W, et al. 2019. Oncoimmunology. 8:e1593807. PubMed
  8. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  9. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  10. Qiu XM, et al. 2020. Reproduction. 251:159. PubMed
  11. Longhi M, et al. 2014. PLoS One. 9:87956. PubMed
  12. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  13. Chandran P, et al. 2014. J Leukoc Biol. 96:633. PubMed
  14. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  15. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  16. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  17. Yan Y, et al. 2018. JCI Insight. 3. PubMed
  18. Zhang H, et al. 2021. Cell Rep. 36:109708. PubMed
  19. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  20. Ishii H, et al. 2022. Cell Rep Med. 3:100520. PubMed
  21. Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed
  22. Almeida VG, et al. 2017. Braz J Med Biol Res. 50:e5163. PubMed
  23. Wing A, et al. 2018. Cancer Immunol Res. 6:605. PubMed
  24. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  25. Teni Nurlatifah HR, et al. 2021. Med Arch. 75:335. PubMed
  26. Zhang W, et al. 2022. J Transl Med. 20:558. PubMed
  27. Guimarães F, et al. 2017. J Pediatr (Rio J). 10.1016/j.jped.2017.08.005. PubMed
  28. Roudko V, et al. 2020. Cell. 183(6):1634-1649.e17. PubMed
  29. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  30. Hr TN, et al. 2021. Allergol Immunopathol (Madr). 49:193. PubMed
  31. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  32. Lu H, et al. 2020. Oncoimmunology. 9:1748991. PubMed
  33. Xian S, et al. 2021. EMBO Rep. 22:e52509. PubMed
  34. Zanetti S, et al. 2016. J Biol Chem. 291: 16541 - 16552. PubMed
  35. Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
  36. Faissner S, et al. 2022. Front Immunol. 13:980526. PubMed
  37. Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
RRID
AB_315233 (BioLegend Cat. No. 502508)
AB_315235 (BioLegend Cat. No. 502510)
AB_315234 (BioLegend Cat. No. 502509)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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