Biotinylated Recombinant Human PD-1-Fc (CD279) Chimera (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
Programmed Cell Death Protein 1, PD1, PDCD1, CD279
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Product Citations
Biotinylated recombinant human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_BIOTIN_RECOM_CF_1_031023
    Biotinylated recombinant human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 - 12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_BIOTIN_RECOM_CF_2_031023
    Stability Testing for Biotinylated Recombinant Human PD-1-Fc. Biotinylated recombinant human PD-1-Fc was aliquoted in PBS, 5% glycerol at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to bind to immobilized recombinant PD-L2 in a dose dependent manner. The ED50 for this effect is 3 -12 ng/mL.
Cat # Size Price Save
799504 25 µg ¥52,740
799506 100 µg ¥134,330

Programmed death-1 (PD-1) is a type I transmembrane protein initially isolated from apoptosis-induced cells by subtractive hybridization. The amino acid sequence of mouse PD-1 shares 59.4% identity to the human counterpart, and a putative tyrosine kinase-association motif is well-conserved. Its extracellular region consists of a single Ig-like variable (IgV) domain. PD-1 is an inhibitory molecule expressed by activated B and T cells and has been implicated in immune tolerance. PD-L1 and PD-L2 are the ligands for PD-1 and their binding leads to the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. PD-1/PD-L1 interaction suppresses immune responses against autoantigens and tumors, and plays an important role in the maintenance of peripheral immune tolerance. Interaction between PD-1 and PD-L1 promote tolerance by blocking TCR-mediated signaling.  PD-L1 expression by tumor cells allow tumor progression by suppressing antitumor T-cell responses. Antibodies against PD-1 or PD-L1 lead to increased antitumor immunity.

Product Details
Technical data sheet

Product Details

Biotinylated Human PD-1, amino acid Leu25-Gln167 (Accession # Q15116.3), with a linker (GS) and a C-terminal human IgG (Pro100-Lys330), followed by a Avi tag, was expressed in 293E cells. Human PD-1-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 391 amino acid recombinant protein has a predicted molecular mass of approximately 43.85 kD. The DTT-reduced and non-reduced protein migrates at approximately 62-70 kD and 120-130 KD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
> 95%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS, 5% glycerol.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Biotinylated Human PD-1-Fc binds to immobilized human PD-L2 at 1 µg/mL (100 µL/well), with an EC50 of 3 -12 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.


Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Antigen Details


Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells,  dendritic cells, and monocytes

Inhibitory receptor on antigen-activated T cells that plays a critical role in induction and maintenance of immune tolerance
APC, monocytes, dendritic cells, stromal cell, cancer cells
B7-H1 (PD-L1, CD274) and B7-DC (PD-L2, CD273)
Measured by its ability to bind to human PD-L2-Fc
Cell Type
Lymphocytes, T cells
Biology Area
Adaptive Immunity, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Ishida Y, et al. 1992. EMBO J. 11:3887-95.
  2. Fife BT, et al. 2009. Nature Immunol. 10:1185-92.
  3. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34.
  4. Latchman Y, et al. 2001. Nat Immunol. 2:261-8.
  5. Iwai Y, et al. 2002. Proc Natl Acad Sci USA. 99:12293-7.
  6. Dai S, et al. 2014. Cell Immunol. 290:72-9.
  7. Tan S, et al. 2017. Nature Commun. 8:14369.
Gene ID
5133 View all products for this Gene ID
View information about PD-1 on

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Go To Top Version: 1    Revision Date: 03/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.


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