APC/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1-4_APCCyanine7_Ly-6C_Antibody_020119
C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • HK1-4_APCCyanine7_Ly-6C_Antibody_020119
    C57BL/6 mouse bone marrow cells were stained with CD8a FITC and Ly-6C (clone HK1.4) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
Cat # Size Price Save
128025 25 µg ¥22,000
128026 100 µg ¥53,000
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  2. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  3. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  4. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  5. Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed
  6. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  7. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  8. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  9. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  10. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  11. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  12. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  13. Simões FC, et al. 2020. Nat Commun. 0.875. PubMed
  14. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  15. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  16. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  17. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  18. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  19. Parsons M, et al. 2014. J Immunol. 192:1361. PubMed
  20. Abdala-Valencia H, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:482. PubMed
  21. Movita D, et al. 2015. J Virol. 89:4809. PubMed
  22. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  23. Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
  24. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  25. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  26. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
  27. Ippagunta S, et al. 2016. Proc Natl Acad Sci U S A. 113: E6162 - E6171. PubMed
  28. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  29. van de Garde M, et al. 2016. PLoS One. 11:e0166094. PubMed
  30. Capuccini B, et al. 2016. Sci Rep. 6:39258. PubMed
  31. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  32. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  33. Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
  34. Grauel AL, et al. 2020. Nat Commun. 4.84375. PubMed
  35. Lynn GM, et al. 2020. Nat Biotechnol. 38:320. PubMed
  36. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  37. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  38. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  39. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  40. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  41. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  42. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  43. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  44. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  45. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
  46. Sun L, et al. 2021. Cancer Cell. :. PubMed
  47. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  48. Motoyama S, et al. 2020. Cells. :9. PubMed
  49. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  50. Zhang X, et al. 2020. Glia. . PubMed
  51. Heng Y, et al. 2020. Glia. . PubMed
  52. Abdul Pari AA, et al. 2020. Cancer Res. 80:2586. PubMed
  53. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  54. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  55. Wang SK, et al. 2021. JCI Insight. 6: . PubMed
RRID
AB_10643867 (BioLegend Cat. No. 128025)
AB_10640120 (BioLegend Cat. No. 128026)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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