APC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_APC_082509
C57BL/6 bone marrow cells stained with HK1.4 APC
  • HK1dot4_APC_082509
    C57BL/6 bone marrow cells stained with HK1.4 APC
Compare all formats See APC spectral data
Cat # Size Price Save
128015 25 µg ¥24,360
128016 100 µg ¥60,060
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Getts D, et al. 2014. Sci Transl Med. 15:219. PubMed
  2. Ray A, et al. 2014. J Immunol. 192:5109. PubMed
  3. Collins C, et al. 2014. Proc Natl Acad Sci U S A. 111:9899. PubMed
  4. Martin R, et al. 2014. J Leukoc Biol. 96:151. PubMed
  5. Pisano F, et al. 2014. PLoS One. 9:103541. PubMed
  6. Sauter K, et al. 2014. J Leukoc Biol. 96:265. PubMed
  7. Price P, et al. 2014. J Virol. 88:10840. PubMed
  8. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  9. Tebartz C, et al. 2015. J Immunol. 194:1100. PubMed
  10. Tanaka T, et al. 2015. J Immunol. 194:2004. PubMed
  11. Rosenheinrich M, et al. 2015. PLoS One. 10: 0136290. PubMed
  12. Kratochvill F, et al. 2015. Cancer Res. 75: 3054-3064. PubMed
  13. Mukovozov I, et al. 2015. J Immunol. 195: 3334 - 3344. PubMed
  14. Radjabova V, et al. 2015. J Immunol. 195: 3149 - 3159. PubMed
  15. Nocera D, et al. 2016. J Immunol. 196: 2860 - 2869. PubMed
  16. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  17. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  18. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  19. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
  20. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
  21. Karki R, et al. 2021. Cell. 184:149. PubMed
  22. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  23. Bordas M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  24. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  25. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  26. Kitamoto S, et al. 2020. Cell. 182(2):447-462.e14. PubMed
  27. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  28. Ohgomori T, et al. 2020. Neuropharmacology. 176:108227. PubMed
  29. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  30. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  31. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  32. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  33. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  34. Pingili AK, et al. 2021. Cell Reports. 35(12):109285. PubMed
  35. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  36. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  37. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  38. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  39. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  40. Kurelac I, et al. 2019. Nat Commun. 10:903. PubMed
  41. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  42. Parlet CP, et al. 2019. Cell Rep. 27:187. PubMed
  43. Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed
  44. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
  45. Christ A, et al. 2018. Cell. 172:162. PubMed
  46. MohanKumar K, et al. 2019. Nat Commun. 10:3494. PubMed
  47. Borggrewe M, et al. 2018. Glia. 66:2645. PubMed
  48. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  49. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  50. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  51. Green DP, et al. 2019. Neuron. 101:412. PubMed
  52. Ohgomori T et al. 2019. J Comp Neurol. 528(1):14-31 . PubMed
  53. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  54. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  55. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  56. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  57. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  58. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  59. Anderson MC, et al. 2018. Cell Chem Biol. 25:483. PubMed
  60. Ghosh S, et al. 2019. Commun Biol. 0.325. PubMed
  61. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  62. Deczkowska A, et al. 2017. Nat Commun. 8:717. PubMed
  63. Pundir P, et al. 2020. Cell Host & Microbe. 26(1):114-122. PubMed
  64. Werneburg S, et al. 2020. Immunity. 52(1):167-182.e7.. PubMed
  65. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
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RRID
AB_1732087 (BioLegend Cat. No. 128015)
AB_1732076 (BioLegend Cat. No. 128016)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/09/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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