APC/Cyanine7 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
N418_APCCyanine7_CD11c_Antibody_022519
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
  • N418_APCCyanine7_CD11c_Antibody_022519
    C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cyanine7 (left) or Armenian hamster IgG APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
117323 25 µg ¥16,740
117324 100 µg ¥51,980
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  2. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  3. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  4. Heindl S, et al. 2021. J Exp Med. 218:. PubMed
  5. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
  6. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  7. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  8. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  9. Huang J, et al. 2022. J Transl Med. 20:496. PubMed
  10. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  11. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  12. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  13. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  14. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  15. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  16. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  17. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  18. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  19. Deslyper G, et al. 2021. Parasitology. 148:539. PubMed
  20. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  21. Sari G, et al. 2020. Biochem Biophys Res Commun. 510:524. PubMed
  22. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  23. Yoshioka D, et al. 2016. Antimicrob Agents Chemother. 60: 6146 - 6154. PubMed
  24. Shon W, et al. 2015. Sci Rep. 5:17305. PubMed
  25. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  26. Casali P, et al. 2021. Front Immunol. 12:761450. PubMed
  27. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  28. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  29. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  30. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  31. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  32. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  33. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  34. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  35. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  36. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  37. Metzger D, et al. 2015. J Infect Dis. 212: 445-452. PubMed
  38. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  39. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  40. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  41. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  42. Trompette A, et al. 2022. Mucosal Immunol. :. PubMed
  43. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  44. Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed
  45. Friedman DJ, et al. 2021. Cancer Immunol Res. 9:952. PubMed
  46. Zhang K, et al. 2014. PLoS Pathog. 10:1004385. PubMed
  47. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  48. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  49. Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed
  50. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  51. Takano T, et al. 2020. Front Immunol. 11:1555. PubMed
  52. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  53. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  54. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  55. Viana C, et al. 2015. PLoS One. 10: 0130809. PubMed
  56. Davis M, et al. 2015. J Immunol. 194:2219. PubMed
  57. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  58. Zerif E, et al. 2020. Int J Mol Sci. 21:. PubMed
  59. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  60. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  61. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  62. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  63. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  64. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  65. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  66. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  67. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  68. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  69. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  70. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  71. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  72. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  73. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  74. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  75. Lee H, et al. 2017. J Immunol. 199:1453. PubMed
  76. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  77. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  78. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  79. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  80. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  81. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  82. Darling R, et al. 2020. Int J Nanomedicine. 5.19375. PubMed
  83. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  84. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  85. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  86. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  87. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  88. Tulyeu J, et al. 2021. Curr Protoc. 1:e283. PubMed
  89. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  90. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  91. Issah Y, et al. 2021. Elife. 10:. PubMed
  92. Konda P, et al. 2022. Am J Cancer Res. 12:210. PubMed
  93. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  94. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  95. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  96. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  97. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  98. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  99. Zhang L, et al. 2020. Cell. 442:181. PubMed
  100. Sheng J, et al. 2021. eLife. 10:00. PubMed
  101. Khan S, et al. 2016. J Exp Med. 213(13):2913-2929. PubMed
  102. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  103. Lau A, et al. 2022. Sci Adv. 8:eabm0142. PubMed
  104. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  105. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
  106. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  107. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  108. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  109. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  110. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  111. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
  112. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  113. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  114. Choi J, et al. 2013. PLoS Negl Trop Dis. 7:e1981. PubMed
RRID
AB_830646 (BioLegend Cat. No. 117323)
AB_830649 (BioLegend Cat. No. 117324)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Spark PLUS UV™ 395 anti-mouse CD11c N418 FC
Go To Top Version: 2    Revision Date: 02/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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