FITC anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_A_FITC_Pos_071006
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_A_FITC_Pos_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
  • N418_B_FITC_Neg_071006
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).
Compare all formats See FITC spectral data
Cat # Size Price Save
117305 50 µg ¥11,880
117306 500 µg ¥25,520
Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  2. Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
  3. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  4. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  5. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  6. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  7. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  8. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  9. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  10. Feola S, et al. 2022. Front Immunol. 13:826164. PubMed
  11. Wilhelm J, et al. 2021. J Control Release. 329:353. PubMed
  12. Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed
  13. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  14. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  15. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  16. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  17. Devenport SN, et al. 2021. JCI Insight. 6: . PubMed
  18. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  19. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  20. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  21. Zhang S, et al. 2022. Acta Pharm Sin B. 12:3124. PubMed
  22. Chen Y, et al. 2018. EBioMedicine. 32:31. PubMed
  23. Zhu H, et al. 2018. Elife. 7:. PubMed
  24. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  25. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  26. Xing J, et al. 2016. Nat Immunol. 17:1373-1380. PubMed
  27. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  28. Pott J et al. 2018. Cell host & microbe. 23(2):191-202 . PubMed
  29. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  30. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  31. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
  32. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  33. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  34. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  35. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  36. Brooks JF, et al. 2020. Cytotherapy. 22:436. PubMed
  37. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  38. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  39. Wu J, et al. 2021. Mol Med Rep. 23:. PubMed
  40. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  41. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  42. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  43. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  44. Shin JE, et al. 2021. Heliyon. 7:e08433. PubMed
  45. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  46. Xiao Y, et al. 2022. Clin Transl Med. 12:e1071. PubMed
  47. Shen J, et al. 2022. Mater Today Bio. 16:100416. PubMed
  48. Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
  49. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  50. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  51. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  52. Jin R, et al. 2020. Mol Cancer Ther. . PubMed
  53. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  54. Ippolito G, et al. 2014. Proc Natl Acad Sci U S A. 111:998. PubMed
  55. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  56. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  57. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  58. Coe D, et al. 2022. JCI Insight. 7:. PubMed
  59. El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed
  60. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  61. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  62. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  63. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  64. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  65. Bharali D, et al. 2008. Nanomedicine. 4:311. PubMed
  66. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  67. Wan X, et al. 2018. Nature. 560:107. PubMed
  68. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  69. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  70. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  71. Mao L, et al. 2022. Front Immunol. 13:833418. PubMed
  72. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
  73. Xue RY, et al. 2019. Front Immunol. 10:1372. PubMed
  74. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  75. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  76. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  77. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  78. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  79. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  80. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  81. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  82. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  83. Han W, et al. 2020. J Immunol. 205:1125. PubMed
  84. Feng Y, et al. 2022. J Nanobiotechnology. 20:193. PubMed
  85. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  86. Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed
  87. Patankar Y, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L902 - L913. PubMed
  88. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  89. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  90. Hsu M, et al. 2022. Nat Immunol. 23:581. PubMed
  91. Miki Y, et al. 2022. JCI Insight. 7:. PubMed
  92. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  93. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  94. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  95. Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
  96. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  97. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  98. Zhang J, et al. 2022. Biomater Res. 26:44. PubMed
  99. Jaiswal M, et al. 2014. J Leukoc Biol. 96:337. PubMed
  100. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  101. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  102. Chin R, et al. 2006. J Immunol. 177:290. PubMed
  103. Gu H, et al. 2021. Elife. 10:. PubMed
  104. Yang Z, et al. 2021. Nat Commun. 12:4299. PubMed
  105. Nasser H, et al. 2020. Cell Death Discov. 0.29375. PubMed
  106. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  107. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  108. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  109. Barrera-Avalos C, et al. 2021. iScience. 24:103520. PubMed
  110. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  111. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  112. Poncini C, et al. 2015. J Immunol. 195: 3311 - 3324. PubMed
  113. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  114. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  115. Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed
  116. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  117. Li Z, et al. 2022. Evid Based Complement Alternat Med. 2022:7421265. PubMed
  118. Wang X, et al. 2022. Elife. 11:. PubMed
  119. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  120. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  121. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  122. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  123. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  124. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  125. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  126. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  127. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  128. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  129. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  130. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  131. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  132. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  133. Lin YC, et al. 2020. Mol Med Rep. 22:4909. PubMed
  134. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
  135. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  136. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  137. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  138. Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
  139. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
RRID
AB_313774 (BioLegend Cat. No. 117305)
AB_313775 (BioLegend Cat. No. 117306)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11c Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD11c N418 FC
Biotin anti-mouse CD11c N418 FC,IHC-F
FITC anti-mouse CD11c N418 FC
PE anti-mouse CD11c N418 FC
Purified anti-mouse CD11c N418 FC,CyTOF®,IP,IHC
Alexa Fluor® 488 anti-mouse CD11c N418 FC
Alexa Fluor® 647 anti-mouse CD11c N418 FC,IHC,ICC,3D IHC
PE/Cyanine5 anti-mouse CD11c N418 FC
PE/Cyanine7 anti-mouse CD11c N418 FC
Brilliant Violet 605™ anti-mouse CD11c N418 FC
Alexa Fluor® 700 anti-mouse CD11c N418 FC
Pacific Blue™ anti-mouse CD11c N418 FC
APC/Cyanine7 anti-mouse CD11c N418 FC
PerCP/Cyanine5.5 anti-mouse CD11c N418 FC
PerCP anti-mouse CD11c N418 FC
Brilliant Violet 421™ anti-mouse CD11c N418 FC,IHC
Brilliant Violet 570™ anti-mouse CD11c N418 FC
Brilliant Violet 785™ anti-mouse CD11c N418 FC
Brilliant Violet 510™ anti-mouse CD11c N418 FC
Brilliant Violet 650™ anti-mouse CD11c N418 FC
Purified anti-mouse CD11c (Maxpar® Ready) N418 FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD11c N418 IHC-F,3D IHC
PE/Dazzle™ 594 anti-mouse CD11c N418 FC
Brilliant Violet 711™ anti-mouse CD11c N418 FC
APC/Fire™ 750 anti-mouse CD11c N418 FC
TotalSeq™-A0106 anti-mouse CD11c N418 PG
Brilliant Violet 750™ anti-mouse CD11c N418 FC
TotalSeq™-B0106 anti-mouse CD11c N418 PG
TotalSeq™-C0106 anti-mouse CD11c N418 PG
KIRAVIA Blue 520™ anti-mouse CD11c N418 FC
Spark Blue™ 550 anti-mouse CD11c N418 FC
Spark NIR™ 685 anti-mouse CD11c N418 FC
Spark UV™ 387 anti-mouse CD11c N418 FC
Spark Red™ 718 anti-mouse CD11c N418 FC
Spark Blue™ 515 anti-mouse CD11c N418 FC
PerCP/Fire™ 806 anti-mouse CD11c N418 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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