- 39-10-8 (See other available formats)
- Regulatory Status
- Other Names
- MHC class II
- Mouse (C3H.SW) IgG3, κ
- Ave. Rating
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- Product Citations
The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- (C3H x BALB/c)F1 mouse cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections.
Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
(PubMed link indicates BioLegend citation)
- Hiramine C, et al. 1995. Cell. Immunol. 160:157.
- Wang Z, et al. 2004. J. Immunol. 172:5924.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed
- Tian C, et al. 2007. J. Immunol. 179:6762.
- Product Citations
AB_493150 (BioLegend Cat. No. 115009)
AB_493149 (BioLegend Cat. No. 115010)
- MHC class II
B cell and activated T cells, APCs of H-2d mice
- Antigen presentation
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Wall KA, et al. 1983. J. Immunol. 131:1056.
4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.
- Gene ID
- 14961 View all products for this Gene ID
- View information about I-Ad on UniProt.org
|Purified anti-mouse I-Ad||39-10-8||FC, IHC-F|
|Biotin anti-mouse I-Ad||39-10-8||FC|
|FITC anti-mouse I-Ad||39-10-8||FC|
|Alexa Fluor® 488 anti-mouse I-Ad||39-10-8||FC|
|Alexa Fluor® 647 anti-mouse I-Ad||39-10-8||FC|
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