Alexa Fluor® 488 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_A488_F4slash80_Antibody_1_FC_060616
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_1_FC_060616
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 Alexa Fluor® 488.
  • BM8_A488_F4slash80_Antibody_2_IHCF_060616
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of anti-mouse F4/80 (clone BM8) Alexa Fluor® 488 (green), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 510™ (blue), and anti-mouse CD3 (clone 17A2) Brilliant Violet 421™ (red) overnight at 4°C. The image was captured with a 10X objective.
See Alexa Fluor® 488 spectral data See high resolution IHC data...
Cat # Size Price Save
123119 25 µg ¥25,000
123120 100 µg ¥55,000
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed
  2. Tedford K, et al. 2017. Nat Commun. . 10.1038/s41467-017-02482-4. PubMed
  3. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  4. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  5. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  6. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  7. Veremeyko T, et al. 2019. J Neurosci Res. 97:162. PubMed
  8. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  9. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  10. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  11. Ooi JD, et al. 2019. Nat Commun. 10:3392. PubMed
  12. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  13. He J, et al. 2019. J Clin Invest. 130. PubMed
  14. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  15. Bordignon P et al. 2019. Cell Rep. 28(9):2358-2372 . PubMed
  16. Luan HH et al. 2019. Cell. 178(5):1231-1244 . PubMed
  17. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
  18. Watanabe M et al. 2017. eLife. 6 pii: e26635. PubMed
  19. Li H, et al. 2019. Front Immunol. 2.205555556. PubMed
  20. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  21. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  22. Settelmeier S, et al. 2020. PLoS One. 15:e0233261. PubMed
  23. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  24. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  25. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  26. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  27. Bian Z, et al. 2012. J Immunol. 188:844. PubMed
  28. Caruso R, et al. 2009. Gastroenterology. 136:2270. PubMed
  29. Carlock C, et al. 2013. Reproduction. 146:491. PubMed
  30. Dong Z, et al. 2013. PLoS One. 18:80364. PubMed
  31. Brocq M, et al. 2014. J Immunol. 192:6120. PubMed
  32. Carlock C, et al. 2014. J Immunol. 193:161. PubMed
  33. Care A, et al. 2014. Biol Reprod. 91:60. PubMed
  34. Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed
  35. Ohnishi A, et al. 2015. PLoS One. 10:117616. PubMed
  36. Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed
  37. Wheeler R, Gull E 2015. Nat Commun. 6: 8964. PubMed
  38. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  39. Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed
  40. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  41. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  42. Niu S, et al. 2016. J Immunol. 197: 3293 - 3301. PubMed
  43. Ho J, et al. 2016. PLoS Biol. 14:e2000117. PubMed
  44. Wang H, et al. 2016. PLoS One. 11:e0167307. PubMed
  45. Yi-Wei Lin, Emmanuel Montassier, Dan Knights 2016. Sci Rep. 6:38599. PubMed
  46. Chakraborty K, et al. 2017. Nat Commun. 8:13944. PubMed
  47. Antsiferova M, et al. 2017. EMBO Mol Med. 9(1):27-45. PubMed
  48. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  49. Ghosh M, et al. 2021. Cancer Cell. 39(4):494-508.e5. PubMed
RRID
AB_893491 (BioLegend Cat. No. 123119)
AB_893479 (BioLegend Cat. No. 123120)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 06/06/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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