- Ber-ACT35 (ACT35) (See other available formats)
- Regulatory Status
- Other Names
- ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
- Mouse IgG1, κ
- Barcode Sequence
- Ave. Rating
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- Product Citations
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CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- HTLV 1-transformed HUT 102 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
- Additional Product Notes
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes.
- Application References
- Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
- Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
- Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
- Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
- Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
- Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
- Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
AB_2892425 (BioLegend Cat. No. 350039)
- 50 kD, TNF receptor family
Activated T cells
- Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
- OX40L (CD252)
- Cell Type
- T cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.
- Gene ID
- 7293 View all products for this Gene ID
- View information about CD134 on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD134 (OX40)
Purified anti-human CD134 (OX40)
PE anti-human CD134 (OX40)
APC anti-human CD134 (OX40)
PerCP/Cyanine5.5 anti-human CD134 (OX40)
PE/Cyanine7 anti-human CD134 (OX40)
Brilliant Violet 421™ anti-human CD134 (OX40)
Purified anti-human CD134 (OX40) (Maxpar® Ready)
Alexa Fluor® 647 anti-human CD134 (OX40)
PE/Dazzle™ 594 anti-human CD134 (OX40)
APC/Cyanine7 anti-human CD134 (OX40)
Biotin anti-human CD134 (OX40)
Brilliant Violet 510™ anti-human CD134 (OX40)
Brilliant Violet 605™ anti-human CD134 (OX40)
APC/Fire™ 750 anti-human CD134 (OX40)
Brilliant Violet 711™ anti-human CD134 (OX40)
TotalSeq™-A0158 anti-human CD134 (OX40)
TotalSeq™-C0158 anti-human CD134 (OX40)
TotalSeq™-B0158 anti-human CD134 (OX40)
TotalSeq™-D0158 anti-human CD134 (OX40)
PE/Cyanine5 anti-human CD134 (OX40)
PE/Fire™ 810 anti-human CD134 (OX40)