Purified anti-human CD283 (TLR3) Antibody

Pricing & Availability
Clone
TLR-104 (See other available formats)
Regulatory Status
RUO
Other Names
Toll like receptor 3, TLR-3, CD283
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
TLR-104_CD283_Purified_Antibody_WB_012114.jpg
Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
  • TLR-104_CD283_Purified_Antibody_WB_012114.jpg
    Western blot analysis of extracts from 293T parent (lane 1) and 293T transfected with human TLR3 (lane 2) using anti-CD283 antibody(TLR-104).
Cat # Size Price Quantity Check Availability Save
315002 100 µg 200,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Toll-like receptor 3 (TLR3) is a 104 kD protein also known as CD283. It is expressed selectively on the cytoplasmic membrane and intracellularly in dendritic cells. TLR3 is also highly expressed in the placenta, pancreas, heart, liver, lung, and muscle. TLR3 is a pattern recognition receptor that participates in innate immune response to microbial pathogens. The ligands recognized by TLR3 are polyinosine-polycytidylic acid (Poly(I:C)) and dsRNA. TLR3 ligands induce NF-κB activation (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 has been shown to interact with a number of proteins including MYD88, TRAF6, TRIAD3, MAP3K7, and TAB2. This antibody is useful for flow cytometry and Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Toll-like receptor 3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC, WB - Quality tested
 

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, suggested working dilution(s)is 10 µg antibody in 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western bloting. The fluorochrome-labeled TLR-104 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis. For intracellular staining protocol, please visit www.biolegend.com and click on the support tab.

Application References
  1. Fiala M, et al. 2007. P. Natl. Acad. Sci. USA 10.1073/P. Natl. Acad. Sci. USA.0701267104.
  2. Kong KF, et al. 2008. J. Virol. 82:7613. PubMed
  3. Sadik CD, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
Product Citations
  1. Kong K, et al. 2008. J Virol. 82:7613. PubMed
  2. Sadik C, et al. 2009. Nucleic Acids Res. 37:5041. PubMed
RRID
AB_2303470 (BioLegend Cat. No. 315002)

Antigen Details

Structure
Toll-like receptor family member, type I membrane protein containing multiple leucine repeats and a interleukin-1 receptor like region in the extracellular domain, predicted molecular weight approximately 104 kD
Distribution

Selective expression in dendritic cells. Highly expressed in placenta, pancreas, also expressed in heart, liver, lung and muscle

Function
Pattern recognition receptor that participates in innate immune response to microbial pathogens. TLR3 ligands induce NF-κB (via TRIF-linked RIP1/TLR3 interactions) and cytokine production. TLR3 stimulation results in the production of the cytokines
Interaction
MYD88, TRAF6, TRIAD3, MAP3K7, TAB2
Ligand/Receptor
Polyinosine-polycytidylic acid (Poly(I:C)), dsRNA
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Alexopoulou L, et al. 2001. Nature 413:732.
2. Doyle SE, et al. 2002. Immunity 17:251.
3. Meylan E, et al. 2004. Nature Immun. 5:503.
4. Muzio M, et al. 2000. J. Immunol. 164:5998.
5. Rock FL, et al. 1998. Proc. Natl. Acad. Sci. 95:588.

Gene ID
7098 View all products for this Gene ID
UniProt
View information about CD283 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account