PerCP/Cyanine5.5 anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
GL3_PCPC5_1_062310
C57BL/6 splenocytes stained with CD3e (145-2C11) FITC and GL3 PerCP/Cyanine5.5
  • GL3_PCPC5_1_062310
    C57BL/6 splenocytes stained with CD3e (145-2C11) FITC and GL3 PerCP/Cyanine5.5
  • GL3_PCPC5_2_062310
    C57BL/6 splenocytes stained with CD3e (145-2C11) FITC and Armenian Hamster IgG PerCP/Cyanine5.5 isotype control
See PerCP/Cyanine5.5 spectral data
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118117 25 µg 80,00€
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118118 100 µg 220,00€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
  2. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  3. Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed
  4. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  5. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  6. Zhang H et al. 2019. Cell Rep. 29(2):391-405 . PubMed
  7. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  8. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  9. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  10. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  11. Jin C, et al. 2019. Cell. 176:998. PubMed
  12. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  13. Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
  14. Babb R, et al. 2016. Clin Sci. 130: 697 - 710. PubMed
  15. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  16. Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
  17. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  18. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  19. Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed
  20. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  21. Polese B, et al. 2021. Cell Reports. 36(4):109456. PubMed
  22. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
RRID
AB_10612572 (BioLegend Cat. No. 118117)
AB_10612756 (BioLegend Cat. No. 118118)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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