APC Anti-mouse TCR γ/δ Antibody

Pricing & Availability
Clone
GL3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor γ/δ
Isotype
Armenian Hamster IgG
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Product Citations
publications
A_GL3_APC_083109
C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and GL-3 APC
  • A_GL3_APC_083109
    C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and GL-3 APC
  • B_GL3_APC_083109.jpg
    C57BL/6 mouse lymph node cells stained with CD3 (145-2C11) PE and Armenian hamster IgG APC isotype control
See APC spectral data
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118115 25 µg 68,00€
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118116 100 µg 112,00€
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4-/CD8- although some are CD8+. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
C57BL/6J intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The GL3 antibody has been shown to be useful in identifying γ/δ T cells by flow cytometry and immunohistochemistry and depleting γ/δ T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ T cells3-5.

Application References
  1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
  2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
  3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
  4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
  5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
  6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
  8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
  9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
Product Citations
  1. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  2. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  3. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  4. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
  5. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  6. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  7. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  8. Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
  9. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  10. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  11. Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
  12. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  13. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  14. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  15. Guo Y, et al. 2013. Infect Immun . 81:3923. PubMed
  16. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  17. Pai M, et al. 2016. JPEN J Parenter Enteral Nutr. 40: 383 - 391. PubMed
  18. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
  19. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  20. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
RRID
AB_1731824 (BioLegend Cat. No. 118115)
AB_1731813 (BioLegend Cat. No. 118116)

Antigen Details

Structure
Ig superfamily, associates with CD3 complex.
Distribution

T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.

Function
Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
Ligand/Receptor
Some bacterial or tumor antigens bound to MHC class I.
Cell Type
Epithelial cells, T cells, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Skarstein K, et al. 1995. Immunology. 81:497.
  2. Harrison LC, et al. 1996. J Exp Med. 184:2167.
  3. Wildner G, et al. 1996. Eur J Immunol. 26:2140.
  4. Brandes M, et al. 2005. Science. 309:264.
Gene ID
110066 View all products for this Gene ID 110067 View all products for this Gene ID
UniProt
View information about TCR gamma/delta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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