PerCP/Cyanine5.5 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_PerCPCyanine55_CD19_Antibody_102318
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • 6D5_PerCPCyanine55_CD19_Antibody_102318
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
115533 25 µg 60,00€
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115534 100 µg 196,00€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  2. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  3. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  4. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  5. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  6. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  7. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  8. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  9. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  10. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  11. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  12. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  13. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  14. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  15. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  16. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  17. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  18. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  19. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  20. Santiago M, et al. 2010. J Immunol. 185:1114. PubMed
  21. Smith D, et al. 2011. PLoS One. 7:e1002284. PubMed
  22. Hajas A, et al. 2013. Hum Immunol. 19:93. PubMed
  23. Li S, et al. 2013. Virology. 443:143. PubMed
  24. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  25. Li S, et al. 2014. J Immunol. 193:306. PubMed
  26. White C, et al. 2015. J Immunol. 194:697. PubMed
  27. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  28. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  29. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  30. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  31. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  32. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  33. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  34. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  35. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  37. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  38. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
RRID
AB_2259869 (BioLegend Cat. No. 115533)
AB_2072925 (BioLegend Cat. No. 115534)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 10/23/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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