FITC anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_
C57BL/6 splenocytes were stained with CD19 (clone 6D5) FITC and CD3 PE.
  • 6D5_
    C57BL/6 splenocytes were stained with CD19 (clone 6D5) FITC and CD3 PE.
See FITC spectral data
Cat # Size Price Quantity Avail. Save
115505 50 µg 36,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115506 500 µg 88,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  2. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  3. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  4. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  5. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  6. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  7. Zhang Y, et al. 2017. J Invest Dermatol. 137:2532. PubMed
  8. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  9. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  10. Das S, et al. 2019. Oncogene. 383316. PubMed
  11. Spangenberg E, et al. 2019. Nat Commun. 10:3758. PubMed
  12. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  13. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  14. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  15. Antonia O Cuff et al. 2017. Wellcome open research. 2:39 . PubMed
  16. Furuhashi K, et al. 2017. Immunology. 151:227. PubMed
  17. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  18. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
  19. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  20. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  21. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  22. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  23. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  24. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  25. Vacca F, et al. 2020. eLife. 9:e54017.. PubMed
  26. Zheng Z, et al. 2020. Cell Reports. 31(13):107819. PubMed
  27. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  28. Sun S, et al. 2013. J Immunol. 190:6209. PubMed
  29. Weinheimer-Haus E, et al. 2014. PLoS One. 9:91355. PubMed
  30. Fan X, et al. 2014. PLoS One. 9:107638. PubMed
  31. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  32. Omenetti S, et al. 2015. Cell Mol Gastroenterol Hepatol. 1: 406-419. PubMed
  33. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  34. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
  35. Ma J, et al. 2016. Proc Natl Acad Sci U S A. 113: 450 - 458. PubMed
  36. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
  37. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  38. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  39. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  40. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
RRID
AB_313640 (BioLegend Cat. No. 115505)
AB_313641 (BioLegend Cat. No. 115506)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/24/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account