Loading...

PE/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5-PEslashCy7
C57BL/6 splenocytes were stained with CD19 (clone 6D5) PE/Cyanine7 and CD3 FITC.
  • 6D5-PEslashCy7
    C57BL/6 splenocytes were stained with CD19 (clone 6D5) PE/Cyanine7 and CD3 FITC.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
115519 25 µg 62€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115520 100 µg 172€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  4. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  5. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  6. Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed
  7. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  8. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  9. d'Errico P, et al. 2021. Nat Neurosci. Online ahead of print. PubMed
  10. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  11. Clark K, et al. 2015. J Autoimmun. Available online 21 August 2015. PubMed
  12. Castañeda-Zegarra S, et al. 2020. Aging (Albany NY). 12:00. PubMed
  13. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  14. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  15. Yang C, et al. 2020. J Control Release. 326:324. PubMed
  16. Lin CA, et al. 2021. Pharmaceutics. 13:. PubMed
  17. Morgado P, et al. 2014. Infect Immun . 82:4047. PubMed
  18. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  19. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  20. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  21. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  22. Alberti D, et al. 2021. Curr Protoc. 1:e311. PubMed
  23. Rogawski DS, et al. 2021. Nat Commun. 12:2792. PubMed
  24. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
  25. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  26. Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed
  27. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  28. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  29. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  30. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  31. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
  32. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  33. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  34. Pereira JA, et al. 2020. Hum Mol Genet. 29:1253. PubMed
  35. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  36. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  37. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  38. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  39. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  40. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  41. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  42. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  43. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  44. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  45. Challa TD, et al. 2020. Cell Reports. 30(10):3424-3433. PubMed
  46. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  47. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  48. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  49. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  50. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  51. Pillonel V, et al. 2016. Sci Rep. 6:37772. PubMed
  52. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  53. Kim MY, et al. 2018. Cell. 173:1439. PubMed
  54. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  55. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  56. Ma K, et al. 2022. iScience. 25:104347. PubMed
  57. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  58. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  59. Wieghofer P, et al. 2021. EMBO J. 40:e105123. PubMed
  60. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  61. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  62. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  63. Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
  64. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  65. Ackermann C, et al. 2019. Sci Rep. 9:10624. PubMed
  66. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  67. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  68. Vincent B, et al. 2010. J Immunol. 184:4196. PubMed
  69. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  70. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  71. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  72. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  73. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  74. Korin B, et al. 2020. Sleep. :43. PubMed
  75. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  76. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  77. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  78. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  79. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  80. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  81. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  82. Bansal K, et al. 2017. Nat Immunol. 18:263-273. PubMed
  83. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  84. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  85. Song W, et al. 2022. Immunity. 55:290. PubMed
  86. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
  87. Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
RRID
AB_313654 (BioLegend Cat. No. 115519)
AB_313655 (BioLegend Cat. No. 115520)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 2    Revision Date: 05/24/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account