PE anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PE_021606
C57BL/6 mouse splenocytes stained with 17A2 PE
  • 17A2_PE_021606
    C57BL/6 mouse splenocytes stained with 17A2 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
100205 50 µg 40€
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100206 200 µg 96€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Pedraza-Zamora C, et al. 2017. Parasite Immunol. 10.1111/pim.12420. PubMed
  2. Xu C, et al. 2017. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2017.05.071. PubMed
  3. Ushijima M, et al. 2018. Front Immunol. 9:243. PubMed
  4. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  5. Sun J et al. 2018. Cell stem cell. 23(3):355-369 . PubMed
  6. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  7. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
  8. Pedraza–Zamora CP, et al. 2017. Parasite Immunol. 39:4. PubMed
  9. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  10. Doonan J, et al. 2019. Nat Commun. 10:1554. PubMed
  11. Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed
  12. Kim JH, et al. 2019. Mol Med. 25:33. PubMed
  13. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  14. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  15. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  16. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  17. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  18. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  19. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  20. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  21. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  22. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  23. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  24. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  25. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  26. Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed
  27. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  28. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  29. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  30. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  31. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  32. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  33. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  34. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  35. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  36. Ding Y, et al. 2020. Evid Based Complement Alternat Med. 2020:9521073. PubMed
  37. Winchell CG, et al. 2020. Front Immunol. 1.077083333. PubMed
  38. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  39. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  40. Uluçkan ö, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
  41. Zhang T, et al. 2020. Cell. 180(6):1115-1129. PubMed
  42. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  43. Paul S, et al. 2018. Appl Microbiol Biotechnol. 102:8895. PubMed
  44. Fathman J, et al. 2010. Proc Natl Acad Sci U S A. 107:3663. PubMed
  45. Bedi B, et al. 2012. Proc Natl Acad Sci U S A. 109:275. PubMed
  46. Hsieh Y, et al. 2014. Infect Immun . 82:2087. PubMed
  47. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  48. Schneider A, et al. 2014. J Leukoc Biol. 96:857. PubMed
  49. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  50. Chen Y, et al. 2015. PLoS One. 10: 0135217. PubMed
  51. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  52. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  53. Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed
  54. Reboldi A, et al. 2016. Sci Transl Med. 352: aaf4822. PubMed
  55. Schmidt D, et al. 2016. PLoS One. 11: 0159059. PubMed
  56. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  57. Yamamura K, et al. 2017. Nat Commun. 8:13946. PubMed
RRID
AB_312662 (BioLegend Cat. No. 100205)
AB_312663 (BioLegend Cat. No. 100206)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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