APC anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
17A2_APC_073012
C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
  • 17A2_APC_073012
    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).
Compare all formats See APC spectral data
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100235 25 µg 27€
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100236 100 µg 62€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
  2. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  3. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  4. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  5. Liu J, et al. 2019. Immunity. 50:600. PubMed
  6. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  7. Morse M, et al. 2016. Lupus. 25: 81 - 87. PubMed
  8. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  9. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
  10. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  11. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  12. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
  13. Naito Y, et al. 2019. American Journal of Physiology-Renal Physiology. 318(1):F238-F247. PubMed
  14. Fu X, et al. 2020. J Immunol. 205:2066. PubMed
  15. Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed
  16. Baram T, et al. 2021. Cells. 10:. PubMed
  17. Luo R, et al. 2021. Theranostics. 11:117. PubMed
  18. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  19. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  20. Wilson RP, et al. 2019. PLoS Pathog. 15:e1007745. PubMed
  21. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  22. Huang X, et al. 2019. Cancer Immunol Res. 1.388888889. PubMed
  23. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  24. Sano T, et al. 2021. Cell Reports. 36(8):109608. PubMed
  25. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  27. Forte E, et al. 2020. J Cell Mol Med. . PubMed
  28. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  29. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  30. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  31. Shi S, et al. 2020. Clin Cancer Res. 26:5990. PubMed
  32. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  33. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  34. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  35. Peng Z, et al. 2021. STAR Protocols. 2(2):100595. PubMed
  36. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  37. Tang D, et al. 2020. Br J Haematol. 191:906. PubMed
  38. van Elsas MJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  39. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
  40. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  41. Mastandrea I, et al. 2022. STAR Protoc. 3:101106. PubMed
  42. Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed
  43. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  44. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  45. Thomas S, et al. 2019. J Immunother Cancer. 7:214. PubMed
  46. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  47. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  48. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  49. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  50. Ren HZ, et al. 2022. J Clin Transl Hepatol. 10:1138. PubMed
  51. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  52. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  53. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  54. Wang W, et al. 2022. Aging Cell. 21:e13630. PubMed
  55. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  56. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  57. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  58. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  59. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  60. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  61. Suryawanshi RK, et al. 2021. Nat Commun. 12:6020. PubMed
  62. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  63. Zhang W, et al. 2019. Oncol Lett. 17:815. PubMed
  64. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  65. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  66. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  67. Court AC, et al. 2020. EMBO Rep. 21:e48052. PubMed
  68. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  69. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  70. Zuo W, et al. 2022. Cell Death Discov. 8:257. PubMed
  71. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  72. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  73. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  74. Huang Z, et al. 2021. Nat Commun. 12:145. PubMed
  75. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
  76. Wang Y, et al. 2021. Front Microbiol. 12:701566. PubMed
  77. Zheng D, et al. 2022. Acta Pharm Sin B. 12:2740. PubMed
  78. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  79. Wen M, et al. 2022. JCI Insight. Online ahead of print. PubMed
  80. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  81. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  82. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  83. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  84. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  85. Xiong W, et al. 2022. Adv Sci (Weinh). 9:e2103029. PubMed
  86. Xiao L, et al. 2021. Clin Cancer Res. 27:4338. PubMed
  87. Huang L, et al. 2021. Breast Cancer Res Treat. Online ahead of print. PubMed
  88. Han F, et al. 2020. Invest Ophthalmol Vis Sci. 61:24. PubMed
  89. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  90. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  91. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  92. Yu H, et al. 2021. Front Oncol. 11:736882. PubMed
  93. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  94. Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
  95. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  96. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  97. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  98. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  99. Johnson DC, et al. 2020. Cell Death Dis. 0.894444444. PubMed
  100. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  101. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  102. Li Z, et al. 2022. Nat Commun. 13:6321. PubMed
  103. Olivo Pimentel V, et al. 2021. J Immunother Cancer. 9:. PubMed
  104. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  105. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  106. Zhang J, et al. 2018. Nature. 553:91. PubMed
  107. Adnan-Awad S, et al. 2020. Leukemia. . PubMed
  108. Liu Y, et al. 2020. Oncol Lett. 2369:20. PubMed
  109. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  110. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  111. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  112. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  113. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  114. Yu M, et al. 2021. Molecular Cell. 81(6):1216-1230.e9. PubMed
  115. Liu P, et al. 2020. Mol Cell. 748:77. PubMed
  116. Olson CA, et al. 2021. Cell Host Microbe. 29:1378. PubMed
  117. Kim S, et al. 2020. Autophagy. :1. PubMed
  118. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  119. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  120. Yoshida S, et al. 2022. PLoS One. 17:e0264317. PubMed
  121. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  122. Kelley WJ, et al. 2022. JCI Insight. :. PubMed
  123. Li K, et al. 2020. Nat Commun. 4.844444444. PubMed
  124. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  125. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  126. Arora S, et al. 2021. Med (N Y). 2:938. PubMed
  127. Li H, et al. 2022. iScience. 25:104481. PubMed
  128. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  129. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  130. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  131. Kujur W, et al. 2020. PLoS Pathog. 16:e1009132. PubMed
  132. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  133. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  134. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  135. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  136. Sartorius R, et al. 2018. Front Immunol. 1.413888889. PubMed
  137. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  138. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  139. Gardenier J, et al. 2017. Nat Commun. 8:14345. PubMed
  140. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  141. Zeng A, et al. 2020. Oncol Rep. . PubMed
  142. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  143. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
  144. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  145. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  146. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  147. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
  148. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  149. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID
AB_2561455 (BioLegend Cat. No. 100235)
AB_2561456 (BioLegend Cat. No. 100236)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Alexa Fluor® 488 anti-mouse CD3 17A2 FC, IHC-F, 3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC, ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC, IHC-F, IP, ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F, FC, 3D IHC, SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC, IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3

  • PE anti-mouse CD3

  • Purified anti-mouse CD3

  • Alexa Fluor® 647 anti-mouse CD3

  • Alexa Fluor® 488 anti-mouse CD3

  • Pacific Blue™ anti-mouse CD3

  • Alexa Fluor® 700 anti-mouse CD3

  • PerCP/Cyanine5.5 anti-mouse CD3

  • PE/Cyanine7 anti-mouse CD3

  • APC/Cyanine7 anti-mouse CD3

  • Brilliant Violet 421™ anti-mouse CD3

  • Brilliant Violet 570™ anti-mouse CD3

  • Brilliant Violet 650™ anti-mouse CD3

  • Brilliant Violet 785™ anti-mouse CD3

  • Brilliant Violet 510™ anti-mouse CD3

  • APC anti-mouse CD3

  • Ultra-LEAF™ Purified anti-mouse CD3

  • Brilliant Violet 605™ anti-mouse CD3

  • Alexa Fluor® 594 anti-mouse CD3

  • Brilliant Violet 711™ anti-mouse CD3

  • Biotin anti-mouse CD3

  • PE/Dazzle™ 594 anti-mouse CD3

  • APC/Fire™ 750 anti-mouse CD3

  • Brilliant Violet 750™ anti-mouse CD3

  • TotalSeq™-A0182 anti-mouse CD3

  • TotalSeq™-B0182 anti-mouse CD3

  • Spark Blue™ 550 anti-mouse CD3

  • Spark NIR™ 685 anti-mouse CD3

  • TotalSeq™-C0182 anti-mouse CD3

  • APC/Fire™ 810 anti-mouse CD3

  • PE/Fire™ 640 anti-mouse CD3

  • Spark YG™ 570 anti-mouse CD3

  • PE/Fire™ 700 anti-mouse CD3

  • PE/Cyanine5 anti-mouse CD3

  • Spark Blue™ 574 anti-mouse CD3 Antibody

  • Spark Violet™ 423 anti-mouse CD3

  • PE/Fire™ 810 anti-mouse CD3

  • Spark Red™ 718 anti-mouse CD3

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