APC/Cyanine7 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
3 reviews
Product Citations
publications
17A2_APCCyanine7_CD3_Antibody_031919_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • 17A2_APCCyanine7_CD3_Antibody_031919_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 FITC and CD3 (clone 17A2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
100221 25 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100222 100 µg 176€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions. The solution is free of unconjugated APC/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
  2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
  4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC-F)
  5. Zan H, et al. 2005. EMBO J. 24:3757.
  6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
  8. Konnecke I, et al. 2014. Bone. PubMed
  9. Steelman A, et al. 2013. J Biol Chem. 16:23776 (ICC)
  10. Shi M, et al. 2006. J Immunol. 176:1724 (ICC)
Product Citations
  1. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  2. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  3. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  4. Voss M, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L188 - L195. PubMed
  5. Wen J, et al. 2015. J Pharmacol Sci. 128: 116-124. PubMed
  6. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  7. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  8. Zhou Y, et al. 2016. Acta Pharmacol Sin. 10.1038/aps.2016.102. PubMed
  9. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  10. Teater M, et al. 2018. Nat Commun. 9:222. PubMed
  11. Revathikumar P, et al. 2018. PLoS One. 13:e0193210. PubMed
  12. Draijer C, et al. 2018. Sci Rep. 8:5105. PubMed
  13. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  14. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  15. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  16. Durgeau A, et al. 2018. Nat Commun. 9:5097. PubMed
  17. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  18. Adane B, et al. 2019. Cell Rep. 27:238. PubMed
  19. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  20. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  21. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  22. Kunimoto H et al. 2018. Cancer cell. 33(1):44-59 . PubMed
  23. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  24. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  25. Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed
Publication Library
RRID
AB_2057374 (BioLegend Cat. No. 100221)
AB_2242784 (BioLegend Cat. No. 100222)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 03/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account