PE anti-mouse CD120b (TNF R Type II/p75) Antibody

Pricing & Availability
TR75-89 (See other available formats)
Regulatory Status
Other Names
Armenian Hamster IgG
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Product Citations
C57BL/6 mouse splenocytes stained with TR75-89 PE
  • TR75-89_PE_083107
    C57BL/6 mouse splenocytes stained with TR75-89 PE
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113405 50 µg 98€
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113406 200 µg 276€
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CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Armenian Hamster
E. coli -expressed mouse Type II TNFR
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1. The TR75-89 antibody does not block TNF-α binding to receptor. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113404) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).

Application References
  1. Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (IP)
  2. Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)
  3. Bell JH, et al. 2007. J. Leukoc. Biol. 82:173. (FC)
Product Citations
  1. Ohta S, et al. 2011. PLoS One. 6:e16182. PubMed
  2. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  3. Baram T, et al. 2021. Cells. 10:. PubMed
  4. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  5. Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed
  6. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  7. Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed
  8. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  9. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  10. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  11. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
AB_2206942 (BioLegend Cat. No. 113405)
AB_2206941 (BioLegend Cat. No. 113406)

Antigen Details

TNFR superfamily, 75 kD
Variety of cell types at low levels
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
TNF-α, LT-α (TNF-β)
Cell Type
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.

Gene ID
21938 View all products for this Gene ID
View information about CD120b on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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