Biotin anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
GK1dot5_Biotin_011108
C57BL/6 mouse splenocytes were stained with biotinylated CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
  • GK1dot5_Biotin_011108
    C57BL/6 mouse splenocytes were stained with biotinylated CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
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100403 50 µg 29€
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100404 500 µg 92€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  2. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  3. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  4. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  5. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  6. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  7. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  8. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  9. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  10. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  11. Bian Y, et al. 2017. PLoS Pathog. 13:e1006384. PubMed
  12. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  13. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  14. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  15. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  16. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  17. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  18. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  19. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  20. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  21. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  22. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  23. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  24. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  25. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  26. Dong S, et al. 2021. Nature. 591:117. PubMed
  27. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  28. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  29. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  30. Opejin A, et al. 2020. Cell Reports. 33(8):108424. PubMed
  31. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  32. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  33. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  34. Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed
  35. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  36. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  37. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  38. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  39. Freise AC, et al. 2017. Mol Imaging Biol. 19:599. PubMed
  40. Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed
  41. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  42. Inamine A, et al. 2012. Clin Immunol. 143:170. PubMed
  43. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  44. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  45. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  46. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  47. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  48. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  49. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  50. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  51. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  52. Koide S, et al. 2022. iScience. 25:103603. PubMed
  53. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  54. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  55. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  56. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  57. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  58. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  59. Poholek CH, et al. 2019. Immunohorizons. 0.259722222. PubMed
  60. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  61. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  62. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  63. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  64. Ho K, et al. 2014. Cell Death Dis. 5:1518. PubMed
  65. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  66. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
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RRID
AB_312688 (BioLegend Cat. No. 100403)
AB_312689 (BioLegend Cat. No. 100404)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD4 GK1.5 FC
Biotin anti-mouse CD4 GK1.5 FC,IHC-F,ICC
FITC anti-mouse CD4 GK1.5 FC,IHC-F,ICC
PE anti-mouse CD4 GK1.5 FC
PE/Cyanine5 anti-mouse CD4 GK1.5 FC
Purified anti-mouse CD4 GK1.5 FC,IHC-F,ICC,IP,Costim,Block,Depletion
PE/Cyanine7 anti-mouse CD4 GK1.5 FC
APC/Cyanine7 anti-mouse CD4 GK1.5 FC
Alexa Fluor® 647 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Alexa Fluor® 488 anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Pacific Blue™ anti-mouse CD4 GK1.5 FC
Alexa Fluor® 700 anti-mouse CD4 GK1.5 FC
PerCP anti-mouse CD4 GK1.5 FC
PerCP/Cyanine5.5 anti-mouse CD4 GK1.5 FC
Brilliant Violet 421™ anti-mouse CD4 GK1.5 FC,ICC,IHC-F
Ultra-LEAF™ Purified anti-mouse CD4 GK1.5 FC,Block,Costim,Depletion,IHC,IP
Alexa Fluor® 594 anti-mouse CD4 GK1.5 IHC-F,FC,ICC,SB
Brilliant Violet 711™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 510™ anti-mouse CD4 GK1.5 FC,IHC-F,ICC
Brilliant Violet 605™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 785™ anti-mouse CD4 GK1.5 FC
PE/Dazzle™ 594 anti-mouse CD4 GK1.5 FC
APC/Fire™ 750 anti-mouse CD4 GK1.5 FC
GoInVivo™ Purified anti-mouse CD4 GK1.5 FC
Brilliant Violet 750™ anti-mouse CD4 GK1.5 FC
Brilliant Violet 650™ anti-mouse CD4 GK1.5 FC
Spark Blue™ 550 anti-mouse CD4 GK1.5 FC
Spark NIR™ 685 anti-mouse CD4 GK1.5 FC
KIRAVIA Blue 520™ anti-mouse CD4 GK1.5 FC
PE/Fire™ 640 anti-mouse CD4 GK1.5 FC
APC/Fire™ 810 anti-mouse CD4 GK1.5 FC
PE/Fire™ 700 anti-mouse CD4 GK1.5 FC
Spark Violet™ 538 anti-mouse CD4 GK1.5 FC
Spark YG™ 593 anti-mouse CD4 GK1.5 FC
Spark Blue™ 574 anti-mouse CD4 Antibody GK1.5 FC
Spark UV™ 387 anti-mouse CD4 GK1.5 FC
Spark Blue™ 515 anti-mouse CD4 GK1.5 FC
Spark PLUS UV™ 395 anti-mouse CD4 GK1.5 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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