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APC anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/11.8 (See other available formats)
Regulatory Status
RUO
Other Names
Ecto-5'-nucleotidase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TYslash11.8_APC_1_060412
C57BL/6 mouse splenocytes were stained with CD3 APC/Cy7 and CD73 (clone TY/11.8) APC (top) or rat IgG1, κ APC isotype control (bottom).
  • TYslash11.8_APC_1_060412
    C57BL/6 mouse splenocytes were stained with CD3 APC/Cy7 and CD73 (clone TY/11.8) APC (top) or rat IgG1, κ APC isotype control (bottom).
  • TYslash11.8_APC_2_060412
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
127209 25 µg 91€
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127210 100 µg 186€
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Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References
  1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
  2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Moor AE et al. 2018. Cell. 175(4):1156-1167 . PubMed
  3. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  4. Sarin S et al. 2018. Neuron. 98(1):109-126 . PubMed
  5. Kleine Holthaus SM, et al. 2018. Mol Ther. 26:1343. PubMed
  6. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
  7. Ortín-Martínez A, et al. 2021. EMBO J. 40:e107264. PubMed
  8. Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed
  9. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  10. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  11. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  12. Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed
  13. Shevach H 2016. J Immunol. 196: 1517 - 1528. PubMed
  14. Vieira JMF, et al. 2022. PLoS One. 17:e0272962. PubMed
RRID
AB_11219400 (BioLegend Cat. No. 127209)
AB_11218786 (BioLegend Cat. No. 127210)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Regulation
Expression is regulated by the development and maturation of leukocytes
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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