- S17011E (See other available formats)
- Regulatory Status
- Other Names
- CD16, CD32
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.Product Details
- Antibody Type
- Host Species
- The antibody was purified by affinity chromatography.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- 0.5 mg/ml
- Storage & Handling
- The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
- Recommended Usage
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS for 5-10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µl, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.
- Application Notes
Clone S17011E blocks both clone 93 and 2.4G2 also raised against mouse CD16/32
- Product Citations
AB_2783137 (BioLegend Cat. No. 156603)
AB_2783138 (BioLegend Cat. No. 156604)
- Ig superfamily, 40-60 kD
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
- Cell Type
- B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
- Biology Area
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14130 View all products for this Gene ID 14131 View all products for this Gene ID
- View information about CD16/32 on UniProt.org
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