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Alexa Fluor® 647 anti-mouse CD73 Antibody

Pricing & Availability
Clone
TY/11.8 (See other available formats)
Regulatory Status
RUO
Other Names
Ecto-5'-nucleotidase
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TYslash11.8_A647_CD73_Antibody_Dot_071108
BALB/c mouse splenocytes stained with TY/11.8 Alexa Fluo® 647 and CD3 (145-2C11) PE
  • TYslash11.8_A647_CD73_Antibody_Dot_071108
    BALB/c mouse splenocytes stained with TY/11.8 Alexa Fluo® 647 and CD3 (145-2C11) PE
  • TYslash11.8_A647_CD73_Antibody_IHC_033017
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of Alexa Fluor® 647 anti-mouse CD73 (clone TY/11.8) (red), 10 µg/ml of Alexa Fluor® 488 anti-mouse CD3 (clone 17A2) (green) and 10 µg/ml of Brilliant Violet 421™ anti-mouse/human B220 (clone RA3-6B2) (blue) overnight at 4°C. The image was captured by 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
127207 25 µg 100€
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127208 100 µg 212€
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Description

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO cells transfected with mouse CD73
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References
  1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
  2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-P)
Product Citations
  1. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  2. Pan X, et al. 2011. PLoS One. 6:e25839. PubMed
  3. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  4. Nakai T, et al. 2021. STAR Protoc. 2:100826. PubMed
RRID
AB_2154093 (BioLegend Cat. No. 127207)
AB_2267373 (BioLegend Cat. No. 127208)

Antigen Details

Structure
69 kD GPI-anchored surface protein
Distribution

Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73

Function
A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor
Nucleoside 5' monophosphate
Cell Type
B cells, Mesenchymal Stem Cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Yamashita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Resta R, et al. 1998. Immunol. Rev. 161:95.

Regulation
Expression is regulated by the development and maturation of leukocytes
Gene ID
23959 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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