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PerCP/Cyanine5.5 anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
Clone
MH5A (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Armenian Hamster IgG
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Product Citations
publications
MH5A_PerCPCy5.5_1_062612
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) PerCP/Cyanine5.5 (top) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (bottom).
  • MH5A_PerCPCy5.5_1_062612
    C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) PerCP/Cyanine5.5 (top) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (bottom).
  • MH5A_PerCPCy5.5_2_062612
Compare all formats See PerCP/Cyanine5.5 spectral data
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143712 100 µg 306€
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
PD-1H- IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
RRID
AB_2561400 (BioLegend Cat. No. 143712)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family
Distribution

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Function
Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about PD-1H on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse PD-1H (VISTA)

  • PE anti-mouse PD-1H (VISTA)

  • FITC anti-mouse PD-1H (VISTA)

  • APC anti-mouse PD-1H (VISTA)

  • PerCP/Cyanine5.5 anti-mouse PD-1H (VISTA)

  • PE/Cyanine7 anti-mouse PD-1H (VISTA)

  • Brilliant Violet 421™ anti-mouse PD-1H (VISTA)

  • Alexa Fluor® 647 anti-mouse PD-1H (VISTA)

  • Alexa Fluor® 488 anti-mouse PD-1H (VISTA)

  • PE/Dazzle™ 594 anti-mouse PD-1H (VISTA)

  • Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA)
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