Brilliant Violet 421™ anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
MH5A (See other available formats)
Regulatory Status
Other Names
PD-1 homolog, Dies1, B7-H5
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
C57BL/6 mouse splenocytes were stained with CD8a APC and PD-1H (clone MH5A) Brilliant Violet 421™ (top) or Armenian Hamster IgG Brilliant Violet 421™ isotype control (bottom).
  • MH5A_BV421_PD-1H_Vista_Antibody_1_060717
    C57BL/6 mouse splenocytes were stained with CD8a APC and PD-1H (clone MH5A) Brilliant Violet 421™ (top) or Armenian Hamster IgG Brilliant Violet 421™ isotype control (bottom).
  • MH5A_BV421_PD-1H_Vista_Antibody_2_060717
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
143715 50 µg 234€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Armenian Hamster
PD-1H- IgG Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
AB_2687302 (BioLegend Cat. No. 143715)

Antigen Details

Type I transmembrane protein, member of the CD28 family

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
View information about PD-1H on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 06/07/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse PD-1H (VISTA)

  • PE anti-mouse PD-1H (VISTA)

  • FITC anti-mouse PD-1H (VISTA)

  • APC anti-mouse PD-1H (VISTA)

  • PerCP/Cyanine5.5 anti-mouse PD-1H (VISTA)

  • PE/Cyanine7 anti-mouse PD-1H (VISTA)

  • Brilliant Violet 421™ anti-mouse PD-1H (VISTA)

  • Alexa Fluor® 647 anti-mouse PD-1H (VISTA)

  • Alexa Fluor® 488 anti-mouse PD-1H (VISTA)

  • PE/Dazzle™ 594 anti-mouse PD-1H (VISTA)

  • Ultra-LEAF™ Purified anti-mouse PD-1H (VISTA)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account