APC anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
MH5A (See other available formats)
Regulatory Status
Other Names
PD-1 homolog, Dies1, B7-H5
Armenian Hamster IgG
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Product Citations
C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) APC (top) or Armenian hamster IgG APC isotype control (bottom).
  • MH5A_APC_1_062612
    C57BL/6 mouse splenocytes were stained with CD8 FITC and PD-1H (clone MH5A) APC (top) or Armenian hamster IgG APC isotype control (bottom).
  • MH5A_APC_2_062612
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
143709 25 µg 124€
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143710 100 µg 300€
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PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Armenian Hamster
PD-1H- IgG Fc fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
AB_11219607 (BioLegend Cat. No. 143709)
AB_11219189 (BioLegend Cat. No. 143710)

Antigen Details

Type I transmembrane protein, member of the CD28 family

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
View information about PD-1H on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 647 anti-mouse PD-1H (VISTA)

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