PE anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
3 reviews
Product Citations
publications
6D5_PE_062310
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 6D5_PE_062310
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data View Moxi Flow Data
Cat # Size Price Quantity Avail. Save
115507 50 µg 28€
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115508 200 µg 88€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Syres K, et al. 2009. Blood. 114:2452. PubMed
  2. Yamaguchi T, et al. 2010. Genes Dev. 24:455. PubMed
  3. Murphy C, et al. 2012. Infect Immun . 80:2712. PubMed
  4. Gorina R, et al. 2014. J Immunol. 192:324. PubMed
  5. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  6. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  7. Muenchow L, et al. 2014. Immunol Lett. 160:113. PubMed
  8. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  9. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  10. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  11. Osaka M, et al. 2016. Sci Rep. 6:21391. PubMed
  12. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  13. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  14. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  15. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  16. Takahashi T, et al. 2017. J Exp Med. 10.1084/jem.20160247. PubMed
  17. Cho S, et al. 2018. Nat Commun. 9:2757. PubMed
  18. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  19. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  20. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  21. Onder L, et al. 2017. Immunity. 47:80. PubMed
  22. Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed
  23. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  24. Yang BH, et al. 2019. Cell Rep. 27:3629. PubMed
Publication Library
RRID
AB_313642 (BioLegend Cat. No. 115507)
AB_313643 (BioLegend Cat. No. 115508)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/24/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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