Loading...

PerCP/Cyanine5.5 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
6D5_PerCPCyanine55_CD19_Antibody_102318
C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
  • 6D5_PerCPCyanine55_CD19_Antibody_102318
    C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD19 (clone 6D5) PerCP/Cyanine5.5 (left), or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
115533 25 µg 72€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115534 100 µg 231€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Fan L, et al. 2020. J Immunol Res. 2020:3032425. PubMed
  2. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  3. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  4. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  5. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  6. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  7. Wei H, et al. 2021. Malar J. 20:89. PubMed
  8. Guzmán M, et al. 2021. Mucosal Immunol. 14:1347. PubMed
  9. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  10. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  11. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  12. Smith D, et al. 2011. PLoS One. 7:e1002284. PubMed
  13. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  14. Gopinathan G, et al. 2022. Mol Cancer Ther. 21:1030. PubMed
  15. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  16. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  17. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  18. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  19. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  20. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  21. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  22. Santiago M, et al. 2010. J Immunol. 185:1114. PubMed
  23. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  24. Yoshihara E, et al. 2020. Nature. 606:586. PubMed
  25. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  26. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  27. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  28. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  29. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  30. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  31. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  32. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  33. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  34. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  35. White C, et al. 2015. J Immunol. 194:697. PubMed
  36. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  37. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  38. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  39. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  40. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  41. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  42. Hajas A, et al. 2013. Hum Immunol. 19:93. PubMed
  43. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  44. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  45. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  46. Glasenapp A, et al. 2021. Circ Res. 129:369. PubMed
  47. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  48. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  49. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  50. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  51. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  52. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  53. Li S, et al. 2013. Virology. 443:143. PubMed
  54. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  55. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  56. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  57. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  58. Li S, et al. 2014. J Immunol. 193:306. PubMed
  59. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  60. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
RRID
AB_2259869 (BioLegend Cat. No. 115533)
AB_2072925 (BioLegend Cat. No. 115534)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 10/23/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account