Ultra-LEAF™ Purified anti-mouse CD152 Antibody

Pricing & Availability
Clone
9H10 (See other available formats)
Regulatory Status
RUO
Other Names
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype
Syrian Hamster IgG
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
106214 25 mg 1408 CHF
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106215 50 mg 2354 CHF
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106216 100 mg 3383 CHF
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106211 100 µg 110 CHF
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106212 1 mg 149 CHF
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106213 5 mg 539 CHF
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Description

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
Mouse CTLA-4-human IgG1 fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

ELISA - Quality tested
FC, Block, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For direct ELISA applications, a concentration range of 1.0 - 8.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of CD152 recombinant protein ranging from 4000 to 62.5 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No, 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106211 - 106216).

Application References

(PubMed link indicates BioLegend citation)
  1. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block Costim FC)
  2. Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
  3. Leach DR, et al. 1996. Science 271:1734. (Block)
  4. Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)
Product Citations
  1. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
RRID
AB_2813942 (BioLegend Cat. No. 106214)
AB_2813943 (BioLegend Cat. No. 106215)
AB_2813944 (BioLegend Cat. No. 106216)
AB_2813939 (BioLegend Cat. No. 106211)
AB_2813940 (BioLegend Cat. No. 106212)
AB_2813941 (BioLegend Cat. No. 106213)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells

Function
Negative regulator of T cell activation
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID
12477 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD152 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
GoInVivo™ Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
Ultra-LEAF™ Purified anti-mouse CD152 9H10 ELISA,FC,Block,Costim
Go To Top Version: 1    Revision Date: 07.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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