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Ultra-LEAF™ Purified anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
F38-2E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
F38-2E2_PE_1_120910
Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • F38-2E2_PE_1_120910
    Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • F38-2E2_PE_2_120910
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345009 100 µg 270 CHF
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345010 1 mg 743 CHF
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Tim-3 fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Costim, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
RRID
AB_11150398 (BioLegend Cat. No. 345009)
AB_11150780 (BioLegend Cat. No. 345010)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD366 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD366 (Tim-3) F38-2E2 FC
PE anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 421™ anti-human CD366 (Tim-3) F38-2E2 FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC anti-human CD366 (Tim-3) F38-2E2 FC
PE/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 605™ anti-human CD366 (Tim-3) F38-2E2 FC
FITC anti-human CD366 (Tim-3) F38-2E2 FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready) F38-2E2 FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3) F38-2E2 FC
APC/Cyanine7 anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 785™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 650™ anti-human CD366 (Tim-3) F38-2E2 FC
Brilliant Violet 510™ anti-human CD366 (Tim-3) F38-2E2 FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3) F38-2E2 FC
GoInVivo™ Purified anti-human CD366 (Tim-3) F38-2E2 FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3) F38-2E2 FC
Pacific Blue™ anti-human CD366 (Tim-3) F38-2E2 FC
Biotin anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-A0169 anti-human CD366 (Tim-3) F38-2E2 PG
TotalSeq™-C0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Cyanine5 anti-human CD366 (Tim-3) F38-2E2 FC
TotalSeq™-B0169 anti-human CD366 (Tim-3) F38-2E2 PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody F38-2E2 FC
TotalSeq™-D0169 anti-human CD366 (Tim-3) F38-2E2 PG
PE/Fire™ 810 anti-human CD366 (Tim-3) F38-2E2 FC
PE/Fire™ 640 anti-human CD366 (Tim-3) F38-2E2 FC
Go To Top Version: 5    Revision Date: 07.03.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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