- ML5 (See other available formats)
- Regulatory Status
- V CD24.5
- Other Names
- Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
- Mouse IgG2a, κ
- Barcode Sequence
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|311149||10 µg||369 CHF|
CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
- The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.
- Additional Product Notes
TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna
The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Application Technical Notes.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
- Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
AB_2922544 (BioLegend Cat. No. 311149)
- GPI-linked glycoprotein, 35-45 kD
B cells, granulocytes, epithelial cells
- B cell proliferation and differentiation
- CD62P (P-Selectin)
- Cell Type
- B cells, Epithelial cells, Granulocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
- Gene ID
- 100133941 View all products for this Gene ID
- View information about CD24 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD24 Reagents Request Custom Conjugation
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD24
PE anti-human CD24
Purified anti-human CD24
Alexa Fluor® 488 anti-human CD24
Alexa Fluor® 647 anti-human CD24
PerCP/Cyanine5.5 anti-human CD24
APC anti-human CD24
PE/Cyanine7 anti-human CD24
Brilliant Violet 421™ anti-human CD24
Brilliant Violet 605™ anti-human CD24
PerCP anti-human CD24
Brilliant Violet 510™ anti-human CD24
Purified anti-human CD24 (Maxpar® Ready)
Biotin anti-human CD24
APC/Cyanine7 anti-human CD24
PE/Dazzle™ 594 anti-human CD24
Brilliant Violet 711™ anti-human CD24
PE anti-human CD24
TotalSeq™-A0180 anti-human CD24
APC/Fire™ 750 anti-human CD24
Brilliant Violet 785™ anti-human CD24
TotalSeq™-C0180 anti-human CD24
TotalSeq™-B0180 anti-human CD24
PE/Cyanine5 anti-human CD24
TotalSeq™-D0180 anti-human CD24
GMP PE anti-human CD24