Purified Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MOPC-21_ChIP_IgG1-k_control_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A) 1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II (clone CTD4H8, Cat. No. 904003) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ isotype control antibody (clone MOPC-21, Cat. No. 400183) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MOPC-21_ChIP_IgG1-k_control_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HeLa cells with either A) 1:300 dilution of Go-ChIP-Grade™ Purified anti-RNA Polymerase II (clone CTD4H8, Cat. No. 904003) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ isotype control antibody (clone MOPC-21, Cat. No. 400183) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human GAPDH gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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400101 50 µg 30 CHF
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400102 500 µg 129 CHF
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC, ICFC - Quality tested
ChIP - Verified
ICC, IHC, IP, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Application References

(PubMed link indicates BioLegend citation)
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  2. Barragué H, et al. 2021. J Immunol. 206:1027. PubMed
  3. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  4. Derakhshandeh R, et al. 2018. Oncotarget. 9:11126. PubMed
  5. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  6. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  7. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  8. Ondigo BN, et al. 2018. Am J Trop Med Hyg. 98:1770. PubMed
  9. Hagemans IM, et al. 2022. J Nanobiotechnology. 20:64. PubMed
  10. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  11. Fraser SD, et al. 2021. ERJ Open Res. :7. PubMed
  12. Lian I, et al. 2011. Placenta. 32:823. PubMed
  13. Smed-Sörensen A, et al. 2008. Blood. 111:5037. PubMed
  14. Oh JH, et al. 2019. Cancer Immunol Res. 0.697222222. PubMed
  15. Mastoridis S, et al. 2021. Am J Transplant. 21:2387. PubMed
  16. Carlsten M, et al. 2007. Cancer Res . 67:1317. PubMed
  17. Nakajima NI, et al. 2017. Oncol Rep. 38:693. PubMed
  18. Zhang Y, et al. 2021. Adv Sci (Weinh). 8:2003380. PubMed
  19. Fujiwara Y, et al. 2021. PLoS One. 16:e0259846. PubMed
  20. Haruna M, et al. 2020. Biol Pharm Bull. 43:399. PubMed
  21. Li H, et al. 2020. Front Immunol. 11:1777. PubMed
  22. álvaro-Benito M, et al. 2020. Cell Mol Immunol. 17:133. PubMed
  23. Zaborowski MP et al. 2019. Cell reports. 27(1):255-268 . PubMed
  24. Khanolkar A, et al. 2020. Immunohorizons. 4:153. PubMed
  25. Pegues M, McWilliams I, Szalai A 2016. PLoS One. 11: 0159453. PubMed
  26. Hyun J, et al. 2019. PLoS Pathog. 15:e1007883. PubMed
  27. Kegler A, et al. 2019. Oncoimmunology. 8:e1621676. PubMed
  28. Iga N, et al. 2019. Cancer Sci. 110:3434. PubMed
  29. Lukácsi S, et al. 2020. PLoS One. 15:e0232432. PubMed
  30. Chowdhury D, et al. 2021. Front Immunol. 12:755961. PubMed
  31. Sundararaj K, et al. 2021. Immunology. 162:418. PubMed
  32. Erlandsson MC, et al. 2022. iScience. 25:105526. PubMed
  33. Logtenberg MEW, et al. 2019. Nat Med. 25:612. PubMed
  34. Erlichman N, et al. 2022. Cancers (Basel). 14:. PubMed
  35. Samoylenko A, et al. 2021. Sci Rep. 11:19594. PubMed
  36. Karlas A, et al. 2016. Nat Commun. 7:11320. PubMed
  37. Whilding LM, et al. 2019. Cancers (Basel). 11:5. PubMed
  38. Watanabe K, et al. 2018. Am J Physiol Cell Physiol. 315:C398. PubMed
  39. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  40. Bufe B, et al. 2015. J Biol Chem. 290:7369. PubMed
  41. Yoshimura K, et al. 2018. J Vet Med Sci. 80:1068. PubMed
  42. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  43. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  44. Obiedat A, et al. 2020. J Mol Med (Berl). 98:135. PubMed
  45. Luan H, et al. 2021. Cancers (Basel). 13:. PubMed
  46. Lugo-Cintron KM, et al. 2020. Lab Chip. 1586:20. PubMed
  47. Kim N, et al. 2020. Cancers (Basel). 12:. PubMed
  48. Farrington LA, et al. 2020. PLoS Pathog. 16:e1008997. PubMed
  49. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  50. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
  51. Kim MH, et al. 2018. Cancer Immunol Res. 0.427083333. PubMed
  52. Nagy-Baló Z, et al. 2021. PLoS One. 16:e0254853. PubMed
  53. Jiang L, et al. 2019. Sci Rep. 9:3705. PubMed
  54. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  55. Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
  56. Takeuchi T, et al. 2020. Plant Cell. 1240:32. PubMed
  57. Hagiwara K, et al. 2020. Nucleic Acid Ther. 30:346. PubMed
  58. Hwang HJ, et al. 2021. Immun Inflamm Dis. 9:274. PubMed
  59. Bokharaie H, et al. 2022. Biomolecules. 12:. PubMed
  60. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
RRID
AB_2891079 (BioLegend Cat. No. 400102)

Antigen Details

Gene ID
NA

Related FAQs

There are no FAQs for this product.

Other Formats

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Description Clone Applications
Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA,ChIP
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Biotin Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
FITC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
PE Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
APC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
FITC Mouse IgG1, κ Isotype Ctrl (FC) MOPC-21 FC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 ICC
Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC) MOPC-21 ICFC
GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC,ICC,IHC,IP,WB,FA
FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl MOPC-21 FC,ICFC
TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl MOPC-21 PG
APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl MOPC-21 FC
Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP FITC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl MOPC-21 SB
Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC
Spark PLUS UV™ 395 Mouse IgG1, κ Isotype Ctrl MOPC-21 FC,ICFC
Go To Top Version: 5    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 510™ Mouse IgG1, κ Isotype Ctrl

  • Purified Mouse IgG1, κ Isotype Ctrl

    MOPC-21_ChIP_IgG1-k_control_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...
  • APC Mouse IgG1, κ Isotype Ctrl

  • Biotin Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine5 Mouse IgG1, κ Isotype Ctrl

  • APC/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • FITC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • PE Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (ICFC)

  • APC Mouse IgG1, κ Isotype Ctrl (FC)

  • PE Mouse IgG1, κ Isotype Ctrl (FC)

  • FITC Mouse IgG1, κ Isotype Ctrl (FC)

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl

  • PerCP Mouse IgG1, κ Isotype Ctrl

    MOPC-21_PerCP_122607
    Human peripheral blood lymphocytes stained with MOPC-21 PerC...
  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 570™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 650™ Mouse IgG1, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 711™ Mouse IgG1, κ Isotype Ctrl

  • Brilliant Violet 785™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 594 Mouse IgG1, κ Isotype Ctrl

  • Alexa Fluor® 700 Mouse IgG1, κ Isotype Ctrl (ICFC)

  • GoInVivo™ Purified Mouse IgG1, κ Isotype Ctrl

  • FITC Mouse IgG1, κ Isotype Ctrl

  • PE Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • APC Mouse IgG1, κ Isotype Ctrl

  • Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-A0090 Mouse IgG1, κ isotype Ctrl

  • Brilliant Violet 750™ Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-B0090 Mouse IgG1, κ isotype Ctrl

  • TotalSeq™-C0090 Mouse IgG1, κ isotype Ctrl

  • APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl

  • Spark NIR™ 685 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 550 Mouse IgG1, κ Isotype Ctrl

  • APC/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 640 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 700 Mouse IgG1, κ isotype Ctrl

  • Spark Violet™ 538 Mouse IgG1, κ isotype Ctrl

  • Spark YG™ 581 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 593 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 423 Mouse IgG1, κ Isotype Ctrl

  • GMP PE Mouse IgG1, κ Isotype Ctrl

  • GMP FITC Mouse IgG1, κ Isotype Ctrl

  • GMP APC Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

  • GMP Pacific Blue™ Mouse IgG1, κ Isotype Ctrl

  • GMP PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl

  • GMP APC/Fire™ 750 Mouse IgG1, κ Isotype Ctrl

  • GMP PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl

  • Spark Violet™ 500 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 810 Mouse IgG1, κ Isotype Ctrl

  • TotalSeq™-Bn0090 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 574 Mouse IgG1, κ Isotype Ctrl

  • Spark YG™ 570 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 806 Mouse IgG1, κ Isotype Ctrl

  • Spark Blue™ 515 Mouse IgG1, κ Isotype Ctrl

  • PerCP/Fire™ 780 Mouse IgG1, κ Isotype Ctrl

  • PE/Fire™ 744 Mouse IgG1, κ Isotype Ctrl

  • Spark UV™ 387 Mouse IgG1, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Mouse IgG1, κ Isotype Ctrl

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