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Purified anti-TCF1 (TCF7) Antibody

Pricing & Availability
Clone
7F11A10 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell-specific transcription factor 1, transcription factor 7, TCF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
7F11A10_TCF1_Purified_Antibody_1_WB_061418_updated
Total cell lysate from Jurkat (lane 1), Molt-4 (lane 2), HeLa (lane 3) and EL4 (lane 4) cell lines (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-TCF1 (TCF7) antibody (clone 7F11A10) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with 1:3000 dilution  of HRP conjugated anti-mouse-IgG secondary antibody for the anti-TCF1 (TCF7) antibody (upper blot) 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower blot). Lane M: Molecular Weight Marker
  • 7F11A10_TCF1_Purified_Antibody_1_WB_061418_updated
    Total cell lysate from Jurkat (lane 1), Molt-4 (lane 2), HeLa (lane 3) and EL4 (lane 4) cell lines (15 µg protein for each) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL (1:250 dilution) of purified anti-TCF1 (TCF7) antibody (clone 7F11A10) (upper blot). Proteins were visualized using chemiluminescence detection after incubation with 1:3000 dilution  of HRP conjugated anti-mouse-IgG secondary antibody for the anti-TCF1 (TCF7) antibody (upper blot) 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower blot). Lane M: Molecular Weight Marker
  • 7F11A10_TCF1_Purified_Antibody_2_IF_090916
    Jurkat cells were fixed with 4% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were stained with 2 µg/mL anti-TCF1 Antibody (clone 7F11A10) followed by Alexa Fluor® 594 (red) conjugated goat anti-mouse IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 7F11A10_TCF1_Purified_Antibody_3_IHC-P_08102022
    IHC staining using purified anti- TCF7 antibody (clone 7F11A10) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 1X Tris-EDTA (final concentration 0.05M), the tissue was incubated with 10 µg/mL of antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The images shown above (left: secondary only control; right: clone 7F11A10 at 10 µg/mL) were captured with a 40X objective. Scalebar = 50 µM.
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655202 100 µg 288 CHF
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Description

TCF1 is the first identified member of the T-cell-specific transcription factor family.  It plays an important role in T cell development and differentiation. TCF1 is inactivated by association with the transcriptional repressor TLE proteins. During Wnt signaling, the transcriptional coactivator CTNNB1 accumulates and, in turn, replaces the transcriptional repressor associated with TCF1. Interaction with CTNNB1 results in transactivation of TCF1 target genes. Deletion of TCF1 causes massive apoptosis of double positive thymocyte, suggesting that TCF1 is required for thymocyte survival during T cell development. In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial TCF1 recombinant protein (116-334 aa)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per mL. For immunocytochemmistry microscopy, a concentration range of 1-5 µg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sekine T, et al. 2020. Sci Immunol. 5: . PubMed
  2. Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed
  3. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  4. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  5. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
RRID
AB_2562103 (BioLegend Cat. No. 655202)

Antigen Details

Structure
384 amino acids, predicted molecular weight of 42 kD. Contains a HMG box DNA binding domain and a CTNNB1 (β-catenin) binding domain.
Distribution

Nucleus

Function
TCF1 is a transcription factor, involved in the canonical Wingless/Integration 1 (Wnt) signaling pathway. TCF1 is essential for survival of CD4+CD8+ double positive thymocytes and differentiation of T cells in the periphery.
Interaction
TCF1 interacts with CTNNB1, TLE1, TLE2, TLE3, TLE4, and AES.
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References

1. Mao CD, et al. 2011. Crit. Rev. Eukaryot. Gene Expr. 21:207.
2. Germar K, et al. 2011. P. Natl. Acad. Sci. USA 108:20060.
3. Wang R, et al. 2011. J. Immunol. 187:5964.
4. Weber BN, et al. 2011. Nature 476:63.
5. Jeannet G, et al. 2010. P. Natl. Acad. Sci. USA 107:9777.
6. Staal FJ, et al. 1999. Int. Immunol. 11:317.

Gene ID
6932 View all products for this Gene ID
UniProt
View information about TCF1 on UniProt.org

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Go To Top Version: 3    Revision Date: 08.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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